Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4487134..4487944 Replicon chromosome
Accession NZ_CP084103
Organism Klebsiella pneumoniae strain C11

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag K8M04_RS21880 Protein ID WP_002887280.1
Coordinates 4487134..4487667 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K8M04_RS21885 Protein ID WP_002887278.1
Coordinates 4487678..4487944 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M04_RS21875 (K8M04_21815) 4485965..4487086 + 1122 WP_142485868.1 cupin domain-containing protein -
K8M04_RS21880 (K8M04_21820) 4487134..4487667 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
K8M04_RS21885 (K8M04_21825) 4487678..4487944 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K8M04_RS21890 (K8M04_21830) 4488047..4489480 - 1434 WP_064172187.1 Cu(+)/Ag(+) sensor histidine kinase -
K8M04_RS21895 (K8M04_21835) 4489470..4490153 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K8M04_RS21900 (K8M04_21840) 4490326..4491711 + 1386 WP_048269805.1 efflux transporter outer membrane subunit -
K8M04_RS21905 (K8M04_21845) 4491729..4492073 + 345 WP_048999680.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T218110 WP_002887280.1 NZ_CP084103:c4487667-4487134 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T218110 NZ_CP115551:874079-874477 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCATCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT218110 WP_002887278.1 NZ_CP084103:c4487944-4487678 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT218110 NZ_CP115551:873852-874079 [Salmonella enterica subsp. enterica serovar Bispebjerg]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References