Detailed information of TA system TA218096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 128226..128851
• Replicon: plasmid unnamed
• Accession: NZ_CP084097
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LCF29_RS25610
• Protein ID: WP_000911333.1
• Coordinates: 128453..128851 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: LCF29_RS25605
• Protein ID: WP_000450520.1
• Coordinates: 128226..128453 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS25605 (128226)	128226..128453	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LCF29_RS25610 (128453)	128453..128851	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LCF29_RS25615 (128860)	128860..131013	-	2154	WP_000009325.1	type IV conjugative transfer system coupling protein TraD	-
LCF29_RS25620 (131266)	131266..131997	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
LCF29_RS25625 (132011)	132011..132520	-	510	WP_021513982.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..148555	148555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T218096 WP_000911333.1 NZ_CP084097:128453-128851 [Escherichia coli O78]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T218096 NZ_CP115463:3418912-3419223 [Caulobacter sp. NIBR1757]
ATGAAAGCTAGACTGTCGGTTCGGGCTCAGCAGGACCTGATCGACATCTACGCCACGGGCGTACGCCTGTTTGGGGTTGC
CCAGGCGGACCGCTATCAGGATGGCTTCGAGGCCGCGTTCGACTTTATCGCTGACTATCCGCTCGCATCGCGGGAACGGG
CCGGCGCCTTTCGATCAGCCAGAATTCATCCCTACAAGTCTCACGTCATCGTCTATCTTCTGGATTCAGACGGCGTGCTG
ATCGTTCGAATCCGGCACGGGCATGAGGATTGGGCGAACGATCCCGCAGGCCCGGACGATCTGAACAACTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT218096 WP_000450520.1 NZ_CP084097:128226-128453 [Escherichia coli O78]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT218096 NZ_CP115463:3418674-3418922 [Caulobacter sp. NIBR1757]
ATGGCGACGATGAATGTCTCCCTGCCCGACGCCATGAAGGATTGGGTCGAGCGCCAAGCGGACGATGGCCGCTACAGCAA
TTCGAGCGACTATGTCCGAGACCTGATCCGCCGGGATCAGGAGCGTACCCGCAAGATCGTGGCCATGAACGCCCTCATTG
AGGAGGGCATCGCCAGTGGCGTTGGCACGATGACGATGGACGAGATCCGGGCCGCTGGTCGGCGCAAGGCCGGGTTGGAT
GAAAGCTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2