Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 115678..116213 | Replicon | plasmid unnamed |
| Accession | NZ_CP084097 | ||
| Organism | Escherichia coli O78 strain 3 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q3YTD6 |
| Locus tag | LCF29_RS25535 | Protein ID | WP_000222766.1 |
| Coordinates | 115678..115965 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | LCF29_RS25540 | Protein ID | WP_001132900.1 |
| Coordinates | 115962..116213 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCF29_RS25530 (114196) | 114196..115409 | + | 1214 | WP_225343800.1 | IS3 family transposase | - |
| LCF29_RS25535 (115678) | 115678..115965 | - | 288 | WP_000222766.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LCF29_RS25540 (115962) | 115962..116213 | - | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| LCF29_RS25545 (117176) | 117176..118033 | - | 858 | WP_000774297.1 | incFII family plasmid replication initiator RepA | - |
| LCF29_RS25550 (118026) | 118026..118508 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| LCF29_RS25555 (118501) | 118501..118548 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| LCF29_RS25560 (118539) | 118539..118790 | + | 252 | WP_223195197.1 | replication protein RepA | - |
| LCF29_RS25565 (118807) | 118807..119064 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| LCF29_RS25570 (119348) | 119348..119497 | - | 150 | WP_001312851.1 | Hok/Gef family protein | - |
| - (119541) | 119541..119602 | + | 62 | NuclAT_1 | - | - |
| - (119541) | 119541..119602 | + | 62 | NuclAT_1 | - | - |
| - (119541) | 119541..119602 | + | 62 | NuclAT_1 | - | - |
| - (119541) | 119541..119602 | + | 62 | NuclAT_1 | - | - |
| LCF29_RS25575 (119858) | 119858..119932 | - | 75 | Protein_128 | endonuclease | - |
| LCF29_RS25580 (120178) | 120178..120390 | - | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| LCF29_RS25585 (120526) | 120526..121086 | - | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sitABCD | iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat | 1..148555 | 148555 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11147.23 Da Isoelectric Point: 10.5832
>T218091 WP_000222766.1 NZ_CP084097:c115965-115678 [Escherichia coli O78]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T218091 NZ_CP115461:c1444195-1443857 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT218091 WP_001132900.1 NZ_CP084097:c116213-115962 [Escherichia coli O78]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT218091 NZ_CP115461:c1443851-1443540 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CDZ2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0VIP2 |