Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 105670..106313 Replicon plasmid unnamed
Accession NZ_CP084097
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LCF29_RS25505 Protein ID WP_001034044.1
Coordinates 105897..106313 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LCF29_RS25500 Protein ID WP_001261286.1
Coordinates 105670..105900 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS25465 (100911) 100911..101078 + 168 Protein_106 colicin-B -
LCF29_RS25470 (101096) 101096..101623 - 528 WP_000203272.1 colicin B immunity protein -
LCF29_RS25475 (101867) 101867..102682 + 816 WP_001312845.1 lipid II-degrading bacteriocin colicin M -
LCF29_RS25480 (102732) 102732..103085 - 354 WP_000864812.1 colicin M immunity protein -
LCF29_RS25485 (103263) 103263..104054 - 792 WP_000016494.1 site-specific integrase -
LCF29_RS25490 (104051) 104051..104740 - 690 WP_000796229.1 hypothetical protein -
LCF29_RS25495 (104784) 104784..105134 - 351 WP_000493379.1 hypothetical protein -
LCF29_RS25500 (105670) 105670..105900 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LCF29_RS25505 (105897) 105897..106313 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LCF29_RS25510 (106388) 106388..107953 + 1566 WP_001128474.1 AAA family ATPase -
LCF29_RS25515 (107938) 107938..108960 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..148555 148555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T218090 WP_001034044.1 NZ_CP084097:105897-106313 [Escherichia coli O78]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T218090 NZ_CP115457:535929-536366 [Sphingobium yanoikuyae]
ATGACCTATCTTCTGGACGTCAATGTACTGGTCGCGCTGATCGACCCCAATCATGTTGGCCATGAAGCGGCCCATCTCTG
GTTTGAAGCCGAAGGCAGTAAAGCGTGGGCGACCTGCCCCATCACCGAAAACGGCGTAATCCGGATCGTTGGCAACGTGC
GCTATCCTTCCACGCCAGGTTCGCCAGCCGAGGTCGCGTCGATCATGGCCCAGATGTGCAGGCTTCCAGGGCACAGCTTC
TGGGCGGAGGACATCAGCCTTCTCAACGCTGACCATGTCGACGCCAGCCAAATCCTCACCTCTGCGCAGGTAACCGACAC
CTTCCTGTTGGCGCTTGCCGTAGCCCGTAAGGGTAAGCTCGCAACCTTCGACCGGCGCCTCACGGCAAGGGGCGTGGCCG
GCGGCAAGGACGCGATCCACCTTATCGGAAATCATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT218090 WP_001261286.1 NZ_CP084097:105670-105900 [Escherichia coli O78]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT218090 NZ_CP115457:535708-535932 [Sphingobium yanoikuyae]
ATGAGAACGACCCTGGTAATCGATGATGACGTGATGGCGGCAGCACGCGCCATTGCCGACCATCAGCACAGCTCCATCGG
CCGCGTCCTGTCCGACCTTGCGCGCAAGGCACTGCATGCGCCAGAGGCATCCCGCACCAGAAATGGCATTAGCCTGCTGC
CGACTAGAGCTGGCGTGGTAGTGACGCAGGACATCGTCAACGCCCTGCGCGATGAGGCGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References