Detailed information of TA system TA218089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 94597..95144
• Replicon: plasmid unnamed
• Accession: NZ_CP084097
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LCF29_RS25430
• Protein ID: WP_097309154.1
• Coordinates: 94597..94875 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: LCF29_RS25435
• Protein ID: WP_000079941.1
• Coordinates: 94875..95144 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS25775 (90861)	90861..90995	-	135	WP_249531455.1	hypothetical protein	-
LCF29_RS25405 (90955)	90955..91896	-	942	WP_001312841.1	3-deoxy-7-phosphoheptulonate synthase	-
LCF29_RS25410 (92077)	92077..92377	+	301	Protein_95	hypothetical protein	-
LCF29_RS25415 (92455)	92455..92802	-	348	WP_000142437.1	DUF6404 family protein	-
LCF29_RS25420 (93104)	93104..93586	-	483	WP_001311056.1	hypothetical protein	-
LCF29_RS25425 (93703)	93703..94551	-	849	WP_001058005.1	3'-5' exonuclease	-
LCF29_RS25430 (94597)	94597..94875	-	279	WP_097309154.1	type II toxin-antitoxin system toxin YacB	Toxin
LCF29_RS25435 (94875)	94875..95144	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
LCF29_RS25440 (95666)	95666..96894	+	1229	WP_112030635.1	IS3-like element IS2 family transposase	-
LCF29_RS25445 (97091)	97091..98365	+	1275	WP_000489607.1	HlyD family secretion protein	-
LCF29_RS25780 (98472)	98472..98744	+	273	Protein_103	cysteine peptidase family C39 domain-containing protein	-
LCF29_RS25455 (98925)	98925..99911	+	987	Protein_104	IS4-like element IS421 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..148555	148555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10799.42 Da
• Isoelectric Point: 8.4615

>T218089 WP_097309154.1 NZ_CP084097:c94875-94597 [Escherichia coli O78]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T218089 NZ_CP115457:532094-532387 [Sphingobium yanoikuyae]
GTGTCACGGCTGATCTGGACGCCGAATGCGCTGGCCGATGTTCAGCGGCTCTATGGTTGGCTCTTGCCCAAGGACGCCGA
AGCCGCGACCCGCGCGGTTGCAACGATCCGGGCCGGGGTCAGGATTCTGGCTGCATCACCGCGCATCGGCCGTCCCGTGG
AGGACATGGACCCTGACTATCGGGAAAAGCTGATCGACTTCGGCAACAGCGGCTATGTCGCCCTCTATCGCCTCGATGGC
GAGACCGTGGCGATCCTAGCCGTCCGCCACCAGAAGGAAGCGGGCTATCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT218089 WP_000079941.1 NZ_CP084097:c95144-94875 [Escherichia coli O78]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT218089 NZ_CP115457:531828-532106 [Sphingobium yanoikuyae]
ATGGCAGCGGCAACATCAATCAAGCTCGATGATGCCCTCAAGGGGCGCGTCCAGCACCTGGCCGAAGTGCGGCGGCGTAG
CTCGCATTGGATCATGCGCGAAGCCATCGCACAATATGTCGAACGTGAGGAAAAGCGCGAGGCGTTCCGGCAGGACGCCA
TACGGGCATGGGATGAATACCAGCAGACGGGCTTGCATCTGACGCTTGAGGAAGCCGACGCCTGGTTGGCGAAGCTCGAA
GCGGGCGAGGATGCGGAACCGCCCAAGTGTCACGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5