Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4684834..4685331 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LCF29_RS22445 Protein ID WP_001173343.1
Coordinates 4684834..4685007 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LCF29_RS22450 Protein ID WP_000398619.1
Coordinates 4685035..4685331 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS22435 (4681504) 4681504..4682961 + 1458 WP_000856836.1 dipeptide/tripeptide permease DtpC -
LCF29_RS22440 (4683198) 4683198..4684715 + 1518 WP_001295065.1 lysine--tRNA ligase -
LCF29_RS22445 (4684834) 4684834..4685007 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LCF29_RS22450 (4685035) 4685035..4685331 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCF29_RS22455 (4685558) 4685558..4685830 - 273 WP_000405653.1 GNAT family N-acetyltransferase -
LCF29_RS22460 (4685842) 4685842..4686072 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LCF29_RS22465 (4686253) 4686253..4687884 + 1632 WP_001216470.1 sensor histidine kinase -
LCF29_RS22470 (4687881) 4687881..4688600 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T218084 WP_001173343.1 NZ_CP084096:c4685007-4684834 [Escherichia coli O78]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T218084 NZ_CP115450:6417803-6418213 [Kitasatospora sp. HUAS 3-15]
GTGATCTATCTCATCGATACCTCCGCATTGTTCCGGCTGATGCGGGATCCGAAGCTCAAGACCGCCTGGTACGACGCGAT
CGACGCAGGGTCGATCGCGTCGTGCTACCCGCAGCGTGCTGAGCTCCTCTTCAGCGCACGCGACGGCCGGGAGTACGACG
AGATCTCCGAGCTGTTCGCTGAGCTCTACCCCGATGTCTCGGTGCCGAAGAACGCAGGACGCTGGATCGACATGGTGCAG
CGGCGGATGGCGCTCGCCGGCCGGCACCGCAGTGCCTCTGCCGTAGACCTGATGATCGCGGCGACGGCCGCGCACCATGG
ACTCACGGTCCTCCACGACGATGCGGACTACCGTACGGTGGCGCGGTACGCCACCGACCTGAGCGAGCACAGCATTCTGG
ACTGCGCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT218084 WP_000398619.1 NZ_CP084096:c4685331-4685035 [Escherichia coli O78]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT218084 NZ_CP115450:6417585-6417806 [Kitasatospora sp. HUAS 3-15]
ATGTCCGTGACCCAGATCGACATCGATGACGAAGCGCTGGAACGCGCAATGGCTCTTGCCGGAGTGCGAACCAAGAAGGA
GGCCGTGAACCTCGCACTGCGCTTCTACGCCGAGCGGCAGGAACGCGCGGCCCGCATCAGTAGGCACTTCGAGCGGGCCC
AGCACTGGGAGGCCCTTGAGCATGCCGAGCGGATGCACCGGGCCGAGAAGGACGCTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References