Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4536484..4537138 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LCF29_RS21685 Protein ID WP_000244777.1
Coordinates 4536484..4536891 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LCF29_RS21690 Protein ID WP_000354046.1
Coordinates 4536872..4537138 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS21665 (4532441) 4532441..4534174 - 1734 WP_225343642.1 single-stranded-DNA-specific exonuclease RecJ -
LCF29_RS21670 (4534180) 4534180..4534890 - 711 WP_000715218.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LCF29_RS21675 (4534915) 4534915..4535811 - 897 WP_000806630.1 site-specific tyrosine recombinase XerD -
LCF29_RS21680 (4535923) 4535923..4536444 + 522 WP_001055874.1 flavodoxin FldB -
LCF29_RS21685 (4536484) 4536484..4536891 - 408 WP_000244777.1 protein YgfX Toxin
LCF29_RS21690 (4536872) 4536872..4537138 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LCF29_RS21695 (4537381) 4537381..4538361 + 981 WP_000886100.1 tRNA-modifying protein YgfZ -
LCF29_RS21705 (4539874) 4539874..4540533 - 660 WP_000250270.1 hemolysin III family protein -
LCF29_RS21710 (4540697) 4540697..4541008 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T218082 WP_000244777.1 NZ_CP084096:c4536891-4536484 [Escherichia coli O78]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T218082 NZ_CP115450:c4665835-4665569 [Kitasatospora sp. HUAS 3-15]
GTGAAGGTCCAGTTCACCGAGCGCGGCTGGAACGACTACCTGTCCTGGCAAGCGGACGACCGCCGCCTCCTCAAGCGCAT
CAACCAGTTGATTGACGACATCCGCCGCAACGGCAACGAGGGCATCGGCAAGCCCGAGCCTTTGCGCCATGAGCTCGCTG
GGGCCTGGTCCCGCCGGATCGACCAGGAGCACCGCCTGGTCTACGTCCTCGACGAGCAAGCCGACACTGTCTGTGTCGTC
GCCTGCCGCTACCACTACAGCAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT218082 WP_000354046.1 NZ_CP084096:c4537138-4536872 [Escherichia coli O78]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT218082 NZ_CP115450:c4666110-4665832 [Kitasatospora sp. HUAS 3-15]
ATGAAGACGATGAGTGCGACTGAACTCCGCGGCAACCTCGCCGCCGCGCTCGACGCCGTCGAGAACGACGCCGAAGAACT
CGTTATCACCCGCGCCGGCCACGAACCGCTGGTCGTCGTCTCCCTCGCCGAGTACGCGTCACTACGCGAGACCGACTACC
TGCTCCGCTCCCCAGCGAACGCCGATCACCTGCGTCGCTCCCTGCAGGAATACCGGGAAGGCCGCGTCCAGGCCCGCGAG
CTGATCGACCCCGACGGCCCCACCGAGCAGACCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References