Detailed information of TA system TA218081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4386736..4387319
• Replicon: chromosome
• Accession: NZ_CP084096
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U9Y5H3
• Locus tag: LCF29_RS21020
• Protein ID: WP_000254735.1
• Coordinates: 4386736..4387071 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U9Y5F1
• Locus tag: LCF29_RS21025
• Protein ID: WP_000581936.1
• Coordinates: 4387071..4387319 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS21005 (4382622)	4382622..4383920	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
LCF29_RS21010 (4384008)	4384008..4385645	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
LCF29_RS21015 (4385873)	4385873..4386664	-	792	WP_001071673.1	nucleoside triphosphate pyrophosphohydrolase	-
LCF29_RS21020 (4386736)	4386736..4387071	-	336	WP_000254735.1	endoribonuclease MazF	Toxin
LCF29_RS21025 (4387071)	4387071..4387319	-	249	WP_000581936.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LCF29_RS21030 (4387397)	4387397..4389631	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
LCF29_RS21035 (4389679)	4389679..4390980	-	1302	WP_225343636.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12070.03 Da
• Isoelectric Point: 8.2605

>T218081 WP_000254735.1 NZ_CP084096:c4387071-4386736 [Escherichia coli O78]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRAKGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T218081 NZ_CP115450:c3307450-3307046 [Kitasatospora sp. HUAS 3-15]
TTGATCTACCTCGACTCGTGTGCGCTGCTGAAGTTCATCAAGCCGGAGCCGGAGACGGCCGCGCTGCGTGCCTGGCGGGA
GGCGCTTCCCGACGGCACCGAGCTGATCACCAGCGAACTGGCCCGGCTGGAGATCACCCGAACGCTGCTCCGCGCGGGCC
TGTCGCACCAGCTCGTCCCGTACCACGTCGACCAAGCGCTTCGGGGCGCCTACCAGGTCGATCTCAGCAGCGCGGTGCTC
GCCAGGGCCCTCGCCTACCGCACCCCGCGGCTGGGATCGCTGGACGCACTCCACCTCGCCAGCGCGGAGCCGTTTCGCGC
CGAGCTCACCGACTTCGTCACCTACGACCGCGAGCTCGCCAGGGCCGCCGAGGAGCTCGGGTTCCCGGTCACGGCCCCCC
GGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9341.71 Da
• Isoelectric Point: 4.4536

>AT218081 WP_000581936.1 NZ_CP084096:c4387319-4387071 [Escherichia coli O78]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHDNIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT218081 NZ_CP115450:c3307728-3307447 [Kitasatospora sp. HUAS 3-15]
ATGGAGACCATGGGACTGCGTGAACTGAACCAGAACCCGTCGAAGGCGGTCGCGCGGGTCCGGGCCGGTGCGACGATCGT
CGTGACCGACCGCGGGCGGCCTGTGATGAGACTCGTGCCGGAGACCGAACCGGAGTCGACCCTCGAACGCATGGTCGCGG
CCGGCGAGGTCACCCCGCCCGCCGAGCAGGGCATGCCCGAGCTCGCCCTCGACCTGCTGCCGGACGTCGGCAGCCTGTCG
GACCTCCTGATCGAAGAGCGGAACCGGGAGCGCGACCGTTGA

Structures

Toxin

• AlphaFold DB: U9Y5H3

Antitoxin

• AlphaFold DB: U9Y5F1