Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4085298..4085923 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LCF29_RS19565 Protein ID WP_000911329.1
Coordinates 4085298..4085696 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LCF29_RS19570 Protein ID WP_000450524.1
Coordinates 4085696..4085923 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS19545 (4081205) 4081205..4081405 + 201 WP_000383836.1 YpfN family protein -
LCF29_RS19550 (4081486) 4081486..4082184 - 699 WP_000679812.1 esterase -
LCF29_RS19555 (4082258) 4082258..4084273 - 2016 WP_000829353.1 tRNA cytosine(34) acetyltransferase TmcA -
LCF29_RS19560 (4084288) 4084288..4085151 - 864 WP_001267498.1 neutral zinc metallopeptidase -
LCF29_RS19565 (4085298) 4085298..4085696 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LCF29_RS19570 (4085696) 4085696..4085923 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LCF29_RS19575 (4086078) 4086078..4086791 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LCF29_RS19580 (4087004) 4087004..4088038 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
LCF29_RS19585 (4088055) 4088055..4088933 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LCF29_RS19590 (4089079) 4089079..4089651 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LCF29_RS19595 (4089651) 4089651..4090121 + 471 WP_001068690.1 thioredoxin-dependent thiol peroxidase -
LCF29_RS19600 (4090374) 4090374..4090904 + 531 WP_225343615.1 4Fe-4S dicluster domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T218080 WP_000911329.1 NZ_CP084096:c4085696-4085298 [Escherichia coli O78]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T218080 NZ_CP084096:c4085696-4085298 [Escherichia coli O78]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT218080 WP_000450524.1 NZ_CP084096:c4085923-4085696 [Escherichia coli O78]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT218080 NZ_CP084096:c4085923-4085696 [Escherichia coli O78]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References