Detailed information of TA system TA218067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2921257..2921895
• Replicon: chromosome
• Accession: NZ_CP084096
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A080J2T0
• Locus tag: LCF29_RS13970
• Protein ID: WP_000813793.1
• Coordinates: 2921257..2921433 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LCF29_RS13975
• Protein ID: WP_001270286.1
• Coordinates: 2921479..2921895 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS13950 (2916879)	2916879..2918051	-	1173	WP_229986595.1	BenE family transporter YdcO	-
LCF29_RS13955 (2918143)	2918143..2918679	+	537	WP_000429150.1	DNA-binding transcriptional regulator SutR	-
LCF29_RS13960 (2918752)	2918752..2920713	+	1962	WP_225343794.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LCF29_RS13965 (2920805)	2920805..2921035	-	231	WP_000494244.1	YncJ family protein	-
LCF29_RS13970 (2921257)	2921257..2921433	+	177	WP_000813793.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LCF29_RS13975 (2921479)	2921479..2921895	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LCF29_RS13980 (2921974)	2921974..2923380	+	1407	WP_000760655.1	PLP-dependent aminotransferase family protein	-
LCF29_RS13985 (2923625)	2923625..2924770	+	1146	WP_225343554.1	ABC transporter substrate-binding protein	-
LCF29_RS13990 (2924788)	2924788..2925801	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
LCF29_RS13995 (2925802)	2925802..2926743	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.83 Da
• Isoelectric Point: 10.9223

>T218067 WP_000813793.1 NZ_CP084096:2921257-2921433 [Escherichia coli O78]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGMRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T218067 NZ_CP115447:3184346-3184657 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT218067 WP_001270286.1 NZ_CP084096:2921479-2921895 [Escherichia coli O78]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT218067 NZ_CP115447:3184026-3184346 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Toxin

• AlphaFold DB: A0A080J2T0