Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1749800..1750418 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LCF29_RS08360 Protein ID WP_001291435.1
Coordinates 1749800..1750018 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LCF29_RS08365 Protein ID WP_000344800.1
Coordinates 1750044..1750418 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS08325 (1745089) 1745089..1745661 + 573 WP_000779831.1 YbaY family lipoprotein -
LCF29_RS08330 (1745692) 1745692..1746003 - 312 WP_000409914.1 MGMT family protein -
LCF29_RS08340 (1746382) 1746382..1746735 + 354 WP_000878146.1 DUF1428 family protein -
LCF29_RS08345 (1746777) 1746777..1748327 - 1551 WP_001362407.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LCF29_RS08350 (1748491) 1748491..1748961 - 471 WP_000136192.1 YlaC family protein -
LCF29_RS08355 (1749077) 1749077..1749628 - 552 WP_000102564.1 maltose O-acetyltransferase -
LCF29_RS08360 (1749800) 1749800..1750018 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LCF29_RS08365 (1750044) 1750044..1750418 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LCF29_RS08370 (1750964) 1750964..1754113 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LCF29_RS08375 (1754136) 1754136..1755329 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218062 WP_001291435.1 NZ_CP084096:c1750018-1749800 [Escherichia coli O78]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T218062 NZ_CP115447:c2904425-2903541 [Mycobacterium tuberculosis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT218062 WP_000344800.1 NZ_CP084096:c1750418-1750044 [Escherichia coli O78]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT218062 NZ_CP115447:c2905315-2904428 [Mycobacterium tuberculosis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTCCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGCCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References