Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1296719..1297269 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag LCF29_RS06290 Protein ID WP_001160974.1
Coordinates 1296955..1297269 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LCF29_RS06285 Protein ID WP_000125566.1
Coordinates 1296719..1296952 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS06260 (1291791) 1291791..1292078 + 288 WP_000203741.1 ferredoxin-like protein FixX -
LCF29_RS06265 (1292137) 1292137..1293468 + 1332 WP_089610134.1 MFS transporter -
LCF29_RS06270 (1293576) 1293576..1294106 + 531 WP_072686445.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LCF29_RS06275 (1294099) 1294099..1295961 + 1863 WP_225343719.1 glutathione-regulated potassium-efflux system protein KefC -
LCF29_RS06280 (1296154) 1296154..1296633 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
LCF29_RS06285 (1296719) 1296719..1296952 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LCF29_RS06290 (1296955) 1296955..1297269 + 315 WP_001160974.1 CcdB family protein Toxin
LCF29_RS06295 (1297266) 1297266..1298114 - 849 WP_000257188.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LCF29_RS06300 (1298121) 1298121..1298498 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LCF29_RS06305 (1298501) 1298501..1299322 - 822 WP_096946043.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LCF29_RS06310 (1299319) 1299319..1300308 - 990 WP_089610127.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LCF29_RS06315 (1300308) 1300308..1301594 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T218060 WP_001160974.1 NZ_CP084096:1296955-1297269 [Escherichia coli O78]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T218060 NZ_CP115447:c2847357-2846962 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT218060 WP_000125566.1 NZ_CP084096:1296719-1296952 [Escherichia coli O78]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT218060 NZ_CP115447:c2846936-2846670 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References