Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1187905..1188481 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag LCF29_RS05760 Protein ID WP_001295743.1
Coordinates 1187905..1188192 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag LCF29_RS05765 Protein ID WP_000063148.1
Coordinates 1188179..1188481 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS05745 (1183556) 1183556..1184869 - 1314 WP_097177238.1 restriction endonuclease subunit S -
LCF29_RS05750 (1184859) 1184859..1186478 - 1620 WP_089625877.1 class I SAM-dependent DNA methyltransferase -
LCF29_RS05755 (1186542) 1186542..1187708 - 1167 WP_000800831.1 restriction endonuclease -
LCF29_RS05760 (1187905) 1187905..1188192 + 288 WP_001295743.1 BrnT family toxin Toxin
LCF29_RS05765 (1188179) 1188179..1188481 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
LCF29_RS05770 (1188515) 1188515..1189471 - 957 WP_089625875.1 GTPase -
LCF29_RS05775 (1189482) 1189482..1189685 - 204 WP_000467859.1 YbdD/YjiX family protein -
LCF29_RS05780 (1189735) 1189735..1191885 - 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
LCF29_RS05785 (1192178) 1192178..1192720 - 543 WP_225343715.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T218059 WP_001295743.1 NZ_CP084096:1187905-1188192 [Escherichia coli O78]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T218059 NZ_CP115447:c2751388-2751065 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT218059 WP_000063148.1 NZ_CP084096:1188179-1188481 [Escherichia coli O78]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT218059 NZ_CP115447:c2751713-2751468 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References