Detailed information of TA system TA218054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 380808..381608
• Replicon: chromosome
• Accession: NZ_CP084096
• Organism: Escherichia coli O78 strain 3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: U9Y257
• Locus tag: LCF29_RS01870
• Protein ID: WP_000342447.1
• Coordinates: 381081..381608 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: U9Y285
• Locus tag: LCF29_RS01865
• Protein ID: WP_001277109.1
• Coordinates: 380808..381074 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF29_RS01845 (376466)	376466..377134	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
LCF29_RS01850 (377127)	377127..378185	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
LCF29_RS01855 (378430)	378430..379284	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LCF29_RS01860 (379555)	379555..380658	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LCF29_RS01865 (380808)	380808..381074	+	267	WP_001277109.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
LCF29_RS01870 (381081)	381081..381608	+	528	WP_000342447.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
LCF29_RS01875 (381605)	381605..381988	-	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LCF29_RS01880 (382412)	382412..383521	+	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LCF29_RS01885 (383569)	383569..384495	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LCF29_RS01890 (384492)	384492..385769	+	1278	WP_000803778.1	branched chain amino acid ABC transporter permease LivM	-
LCF29_RS01895 (385766)	385766..386533	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.63 Da
• Isoelectric Point: 7.0284

>T218054 WP_000342447.1 NZ_CP084096:381081-381608 [Escherichia coli O78]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T218054 NZ_CP115447:c2630525-2630106 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.37 Da
• Isoelectric Point: 10.0863

>AT218054 WP_001277109.1 NZ_CP084096:380808-381074 [Escherichia coli O78]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT218054 NZ_CP115447:c2630746-2630522 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LUM2

Antitoxin

• AlphaFold DB: A0A7U9LUN0