Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 86422..87254 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag LCF29_RS00510 Protein ID WP_000854753.1
Coordinates 86422..86796 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID Q5I3J0
Locus tag LCF29_RS00515 Protein ID WP_001280951.1
Coordinates 86886..87254 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS00480 (81788) 81788..82711 - 924 WP_000535963.1 carboxylate/amino acid/amine transporter -
LCF29_RS00485 (82822) 82822..84006 - 1185 WP_203384730.1 sugar efflux transporter -
LCF29_RS00490 (84403) 84403..84564 - 162 Protein_97 virulence RhuM family protein -
LCF29_RS00495 (84798) 84798..85643 - 846 WP_001406222.1 DUF4942 domain-containing protein -
LCF29_RS00500 (85728) 85728..85925 - 198 WP_000839243.1 DUF957 domain-containing protein -
LCF29_RS00505 (85937) 85937..86425 - 489 WP_001549830.1 DUF5983 family protein -
LCF29_RS00510 (86422) 86422..86796 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
LCF29_RS00515 (86886) 86886..87254 - 369 WP_001280951.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LCF29_RS00520 (87417) 87417..87638 - 222 WP_001405861.1 DUF987 domain-containing protein -
LCF29_RS00525 (87707) 87707..88183 - 477 WP_001186724.1 RadC family protein -
LCF29_RS00530 (88199) 88199..88684 - 486 WP_001469549.1 antirestriction protein -
LCF29_RS00535 (88739) 88739..89557 - 819 WP_001234620.1 DUF932 domain-containing protein -
LCF29_RS00540 (89657) 89657..89890 - 234 WP_001119729.1 DUF905 family protein -
LCF29_RS00545 (89969) 89969..90424 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T218052 WP_000854753.1 NZ_CP084096:c86796-86422 [Escherichia coli O78]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T218052 NZ_CP115447:c2181675-2181358 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13699.56 Da        Isoelectric Point: 7.0268

>AT218052 WP_001280951.1 NZ_CP084096:c87254-86886 [Escherichia coli O78]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT218052 NZ_CP115447:c2181887-2181672 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB Q5I3J0

References