Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4060271..4060889 Replicon chromosome
Accession NZ_CP084055
Organism Escherichia coli strain X14-3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LCR13_RS19795 Protein ID WP_001291435.1
Coordinates 4060271..4060489 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LCR13_RS19800 Protein ID WP_000344800.1
Coordinates 4060515..4060889 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCR13_RS19760 (4055559) 4055559..4056131 + 573 WP_000779831.1 YbaY family lipoprotein -
LCR13_RS19765 (4056162) 4056162..4056473 - 312 WP_000409911.1 MGMT family protein -
LCR13_RS19775 (4056852) 4056852..4057205 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LCR13_RS19780 (4057247) 4057247..4058797 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LCR13_RS19785 (4058961) 4058961..4059431 - 471 WP_000136192.1 YlaC family protein -
LCR13_RS19790 (4059548) 4059548..4060099 - 552 WP_000102564.1 maltose O-acetyltransferase -
LCR13_RS19795 (4060271) 4060271..4060489 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LCR13_RS19800 (4060515) 4060515..4060889 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LCR13_RS19805 (4061435) 4061435..4064584 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LCR13_RS19810 (4064607) 4064607..4065800 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218049 WP_001291435.1 NZ_CP084055:c4060489-4060271 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T218049 NZ_CP115447:2028115-2028513 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT218049 WP_000344800.1 NZ_CP084055:c4060889-4060515 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT218049 NZ_CP115447:2027887-2028114 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References