Detailed information of TA system TA218047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3507563..3508139
• Replicon: chromosome
• Accession: NZ_CP084055
• Organism: Escherichia coli strain X14-3

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LCR13_RS17220
• Protein ID: WP_254825339.1
• Coordinates: 3507563..3507850 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: LCR13_RS17225
• Protein ID: WP_000063160.1
• Coordinates: 3507837..3508139 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCR13_RS17205 (3503800)	3503800..3505080	-	1281	WP_001037390.1	DUF445 domain-containing protein	-
LCR13_RS17210 (3505121)	3505121..3506353	-	1233	WP_001137031.1	multidrug efflux MFS transporter MdtM	-
LCR13_RS17215 (3506668)	3506668..3507364	+	697	Protein_3366	IS1 family transposase	-
LCR13_RS17220 (3507563)	3507563..3507850	+	288	WP_254825339.1	BrnT family toxin	Toxin
LCR13_RS17225 (3507837)	3507837..3508139	+	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
LCR13_RS17230 (3508173)	3508173..3509129	-	957	WP_001297640.1	GTPase	-
LCR13_RS17235 (3509140)	3509140..3509343	-	204	WP_000467859.1	YbdD/YjiX family protein	-
LCR13_RS17240 (3509393)	3509393..3511543	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3507155..3507364	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11353.81 Da
• Isoelectric Point: 6.4877

>T218047 WP_254825339.1 NZ_CP084055:3507563-3507850 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYDIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T218047 NZ_CP115447:c2003143-2002583 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT218047 WP_000063160.1 NZ_CP084055:3507837-3508139 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT218047 NZ_CP115447:c2003481-2003140 [Mycobacterium tuberculosis]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A080I7H7