Detailed information of TA system TA218046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3430289..3430884
• Replicon: chromosome
• Accession: NZ_CP084055
• Organism: Escherichia coli strain X14-3

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: LCR13_RS16825
• Protein ID: WP_000239581.1
• Coordinates: 3430534..3430884 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: LCR13_RS16820
• Protein ID: WP_001223213.1
• Coordinates: 3430289..3430540 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCR13_RS16810 (3425954)	3425954..3429733	+	3780	WP_254825334.1	autotransporter assembly complex protein TamB	-
LCR13_RS16815 (3429736)	3429736..3430077	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LCR13_RS16820 (3430289)	3430289..3430540	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LCR13_RS16825 (3430534)	3430534..3430884	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
LCR13_RS16830 (3430963)	3430963..3431493	-	531	WP_000055072.1	inorganic diphosphatase	-
LCR13_RS16835 (3431803)	3431803..3432759	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LCR13_RS16840 (3432897)	3432897..3434399	+	1503	WP_001095667.1	sugar ABC transporter ATP-binding protein	-
LCR13_RS16845 (3434413)	3434413..3435435	+	1023	WP_001548970.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T218046 WP_000239581.1 NZ_CP084055:3430534-3430884 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T218046 NZ_CP115447:c1995676-1995257 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT218046 WP_001223213.1 NZ_CP084055:3430289-3430540 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT218046 NZ_CP115447:c1995945-1995685 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7