Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3326548..3327045 Replicon chromosome
Accession NZ_CP084055
Organism Escherichia coli strain X14-3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LCR13_RS16295 Protein ID WP_001173343.1
Coordinates 3326872..3327045 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag LCR13_RS16290 Protein ID WP_001425728.1
Coordinates 3326548..3326844 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCR13_RS16270 (3323279) 3323279..3323998 - 720 WP_000611288.1 two-component system response regulator DcuR -
LCR13_RS16275 (3323995) 3323995..3325626 - 1632 WP_001216486.1 sensor histidine kinase -
LCR13_RS16280 (3325807) 3325807..3326037 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
LCR13_RS16285 (3326049) 3326049..3326321 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
LCR13_RS16290 (3326548) 3326548..3326844 + 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCR13_RS16295 (3326872) 3326872..3327045 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LCR13_RS16300 (3327164) 3327164..3328681 - 1518 WP_001295074.1 lysine--tRNA ligase -
LCR13_RS16305 (3328918) 3328918..3330375 - 1458 WP_097330815.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T218045 WP_001173343.1 NZ_CP084055:3326872-3327045 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T218045 NZ_CP115447:c1971081-1970785 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT218045 WP_001425728.1 NZ_CP084055:3326548-3326844 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT218045 NZ_CP115447:c1971329-1971078 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References