Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2052371..2053025 Replicon chromosome
Accession NZ_CP084055
Organism Escherichia coli strain X14-3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LCR13_RS10090 Protein ID WP_000244777.1
Coordinates 2052371..2052778 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LCR13_RS10095 Protein ID WP_000354046.1
Coordinates 2052759..2053025 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCR13_RS10070 (2048328) 2048328..2050061 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
LCR13_RS10075 (2050067) 2050067..2050777 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LCR13_RS10080 (2050802) 2050802..2051698 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LCR13_RS10085 (2051810) 2051810..2052331 + 522 WP_001055874.1 flavodoxin FldB -
LCR13_RS10090 (2052371) 2052371..2052778 - 408 WP_000244777.1 protein YgfX Toxin
LCR13_RS10095 (2052759) 2052759..2053025 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LCR13_RS10100 (2053268) 2053268..2054248 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LCR13_RS10105 (2054444) 2054444..2055103 - 660 WP_000250274.1 hemolysin III family protein -
LCR13_RS10110 (2055267) 2055267..2055578 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LCR13_RS10115 (2055623) 2055623..2057056 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
LCR13_RS10120 (2057113) 2057113..2057856 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T218040 WP_000244777.1 NZ_CP084055:c2052778-2052371 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T218040 NZ_CP115447:1547475-1547879 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT218040 WP_000354046.1 NZ_CP084055:c2053025-2052759 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT218040 NZ_CP115447:1547266-1547469 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References