Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 517325..517963 Replicon chromosome
Accession NZ_CP084055
Organism Escherichia coli strain X14-3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LCR13_RS02600 Protein ID WP_000813794.1
Coordinates 517325..517501 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LCR13_RS02605 Protein ID WP_001270286.1
Coordinates 517547..517963 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCR13_RS02580 (512944) 512944..514119 - 1176 WP_001236251.1 BenE family transporter YdcO -
LCR13_RS02585 (514211) 514211..514747 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
LCR13_RS02590 (514820) 514820..516781 + 1962 WP_223042296.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LCR13_RS02595 (516873) 516873..517103 - 231 WP_000494244.1 YncJ family protein -
LCR13_RS02600 (517325) 517325..517501 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LCR13_RS02605 (517547) 517547..517963 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LCR13_RS02610 (518042) 518042..519448 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
LCR13_RS02615 (519693) 519693..520838 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LCR13_RS02620 (520856) 520856..521869 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
LCR13_RS02625 (521870) 521870..522811 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 511639..512904 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T218029 WP_000813794.1 NZ_CP084055:517325-517501 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T218029 NZ_CP115447:676228-676635 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT218029 WP_001270286.1 NZ_CP084055:517547-517963 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT218029 NZ_CP115447:676016-676231 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References