Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5300987..5301612 Replicon chromosome
Accession NZ_CP084044
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KWJ12_RS25835 Protein ID WP_002882817.1
Coordinates 5300987..5301370 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KWJ12_RS25840 Protein ID WP_004150355.1
Coordinates 5301370..5301612 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWJ12_RS25820 (5298353) 5298353..5299255 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KWJ12_RS25825 (5299252) 5299252..5299887 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KWJ12_RS25830 (5299884) 5299884..5300813 + 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
KWJ12_RS25835 (5300987) 5300987..5301370 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KWJ12_RS25840 (5301370) 5301370..5301612 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KWJ12_RS25845 (5301817) 5301817..5302734 + 918 WP_004181612.1 alpha/beta hydrolase -
KWJ12_RS25850 (5302748) 5302748..5303689 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KWJ12_RS25855 (5303734) 5303734..5304171 - 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
KWJ12_RS25860 (5304168) 5304168..5305028 - 861 WP_004146232.1 virulence factor BrkB family protein -
KWJ12_RS25865 (5305022) 5305022..5305621 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T218024 WP_002882817.1 NZ_CP084044:c5301370-5300987 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T218024 NZ_CP115447:361803-362105 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT218024 WP_004150355.1 NZ_CP084044:c5301612-5301370 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT218024 NZ_CP115447:361579-361806 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGAGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References