Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4682553..4683363 Replicon chromosome
Accession NZ_CP084044
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KWJ12_RS22955 Protein ID WP_004178461.1
Coordinates 4682553..4683086 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KWJ12_RS22960 Protein ID WP_002887278.1
Coordinates 4683097..4683363 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWJ12_RS22950 (4681384) 4681384..4682505 + 1122 WP_004214138.1 cupin domain-containing protein -
KWJ12_RS22955 (4682553) 4682553..4683086 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KWJ12_RS22960 (4683097) 4683097..4683363 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KWJ12_RS22965 (4683466) 4683466..4684899 - 1434 WP_064162551.1 Cu(+)/Ag(+) sensor histidine kinase -
KWJ12_RS22970 (4684889) 4684889..4685572 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KWJ12_RS22975 (4685744) 4685744..4687129 + 1386 WP_064162550.1 efflux transporter outer membrane subunit -
KWJ12_RS22980 (4687147) 4687147..4687491 + 345 WP_064162549.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T218022 WP_004178461.1 NZ_CP084044:c4683086-4682553 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T218022 NZ_CP115396:2588059-2588385 [Hymenobacter yonginensis]
ATGGTAGCAATTTCCCGGTTTGAGGTATGGCTCGTAAACCTTGACCCCACCCAAGGTAGCGAAATCAACAAAACCCGCCC
GTGCGTTATCATCTCTCCGAATGAGCTGAACCGCCACCTGCGCACCGTCATTATTGCAGCCCTGACCAGCACCCGCCGCA
ACTACCCTTCGCGAGTGAACTGTGAGTTTGAGGCCAAAGAAGGGCAGGTAGCGCTGGACCAAATCCGCTCAGTGGACAAA
ACCCGGCTGATGCGGAAGCTAGGGACACTGCCGGATTCAGCTGCCCAGGAGATTTGTGCAGTGCTGCAGGAGTTGTTTCA
GTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT218022 WP_002887278.1 NZ_CP084044:c4683363-4683097 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT218022 NZ_CP115396:2587811-2588065 [Hymenobacter yonginensis]
ATGCAAACCCGCATCATCCGGGTAGGCAATTCCCAGGGGATTGTGCTACCGAAGAAACTGCTGCAACAATATCATCTGTC
GGGCGAAGTAGATTTGCGGGCTACTCCGGATGGCCTGCTGATTACGCCCGTGCTGCGGCCGGCCCGCCAGGATTGGGACG
CCCGCTTTGCCGCGGCAACCGCCGCCGGCGAAGAGCCGGAGGAAGAGATGCTGGAAGGGTTTGCCGATGCCTCTGAAGAC
GAGTGGACATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References