Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4077181..4077800 Replicon chromosome
Accession NZ_CP084044
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KWJ12_RS20030 Protein ID WP_002892050.1
Coordinates 4077582..4077800 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KWJ12_RS20025 Protein ID WP_002892066.1
Coordinates 4077181..4077555 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWJ12_RS20015 (4072333) 4072333..4073526 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KWJ12_RS20020 (4073549) 4073549..4076695 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KWJ12_RS20025 (4077181) 4077181..4077555 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KWJ12_RS20030 (4077582) 4077582..4077800 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KWJ12_RS20035 (4077963) 4077963..4078529 + 567 WP_002892030.1 maltose O-acetyltransferase -
KWJ12_RS20040 (4078501) 4078501..4078641 - 141 WP_004147370.1 hypothetical protein -
KWJ12_RS20045 (4078662) 4078662..4079132 + 471 WP_002892026.1 YlaC family protein -
KWJ12_RS20050 (4079107) 4079107..4080558 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
KWJ12_RS20055 (4080659) 4080659..4081357 + 699 WP_004177238.1 GNAT family protein -
KWJ12_RS20060 (4081354) 4081354..4081494 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KWJ12_RS20065 (4081494) 4081494..4081757 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T218021 WP_002892050.1 NZ_CP084044:4077582-4077800 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T218021 NZ_CP115396:2587811-2588065 [Hymenobacter yonginensis]
ATGCAAACCCGCATCATCCGGGTAGGCAATTCCCAGGGGATTGTGCTACCGAAGAAACTGCTGCAACAATATCATCTGTC
GGGCGAAGTAGATTTGCGGGCTACTCCGGATGGCCTGCTGATTACGCCCGTGCTGCGGCCGGCCCGCCAGGATTGGGACG
CCCGCTTTGCCGCGGCAACCGCCGCCGGCGAAGAGCCGGAGGAAGAGATGCTGGAAGGGTTTGCCGATGCCTCTGAAGAC
GAGTGGACATGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT218021 WP_002892066.1 NZ_CP084044:4077181-4077555 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT218021 NZ_CP115396:2588059-2588385 [Hymenobacter yonginensis]
ATGGTAGCAATTTCCCGGTTTGAGGTATGGCTCGTAAACCTTGACCCCACCCAAGGTAGCGAAATCAACAAAACCCGCCC
GTGCGTTATCATCTCTCCGAATGAGCTGAACCGCCACCTGCGCACCGTCATTATTGCAGCCCTGACCAGCACCCGCCGCA
ACTACCCTTCGCGAGTGAACTGTGAGTTTGAGGCCAAAGAAGGGCAGGTAGCGCTGGACCAAATCCGCTCAGTGGACAAA
ACCCGGCTGATGCGGAAGCTAGGGACACTGCCGGATTCAGCTGCCCAGGAGATTTGTGCAGTGCTGCAGGAGTTGTTTCA
GTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References