Detailed information of TA system TA218019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2741443..2743031
• Replicon: chromosome
• Accession: NZ_CP084044
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KWJ12_RS13525
• Protein ID: WP_064162061.1
• Coordinates: 2741443..2742765 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: KWJ12_RS13530
• Protein ID: WP_002904790.1
• Coordinates: 2742765..2743031 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWJ12_RS13510 (2736499)	2736499..2739000	+	2502	WP_064162059.1	fimbria/pilus outer membrane usher protein	-
KWJ12_RS13515 (2738988)	2738988..2739992	+	1005	WP_064162060.1	fimbrial protein	-
KWJ12_RS13520 (2740134)	2740134..2741426	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KWJ12_RS13525 (2741443)	2741443..2742765	-	1323	WP_064162061.1	type II toxin-antitoxin system HipA family toxin	Toxin
KWJ12_RS13530 (2742765)	2742765..2743031	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KWJ12_RS13535 (2743297)	2743297..2743623	-	327	WP_064162062.1	hydroxyisourate hydrolase	-
KWJ12_RS13540 (2743620)	2743620..2744120	-	501	WP_064162063.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KWJ12_RS13545 (2744098)	2744098..2745477	-	1380	WP_040245186.1	nucleobase:cation symporter-2 family protein	-
KWJ12_RS13550 (2745823)	2745823..2746977	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KWJ12_RS13555 (2746958)	2746958..2747881	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49155.46 Da
• Isoelectric Point: 7.5357

>T218019 WP_064162061.1 NZ_CP084044:c2742765-2741443 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T218019 NZ_CP115307:239884-240234 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT218019 WP_002904790.1 NZ_CP084044:c2743031-2742765 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT218019 NZ_CP115307:239592-239879 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8