Detailed information of TA system TA218010

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 791112..791769
• Replicon: chromosome
• Accession: NZ_CP084044
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KWJ12_RS03945
• Protein ID: WP_040245400.1
• Coordinates: 791359..791769 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: KWJ12_RS03940
• Protein ID: WP_002916312.1
• Coordinates: 791112..791378 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWJ12_RS03915 (786268)	786268..787701	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
KWJ12_RS03920 (787820)	787820..788548	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
KWJ12_RS03925 (788598)	788598..788909	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
KWJ12_RS03930 (789073)	789073..789732	+	660	WP_048334397.1	hemolysin III family protein	-
KWJ12_RS03935 (789883)	789883..790866	-	984	WP_032430613.1	tRNA-modifying protein YgfZ	-
KWJ12_RS03940 (791112)	791112..791378	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
KWJ12_RS03945 (791359)	791359..791769	+	411	WP_040245400.1	protein YgfX	Toxin
KWJ12_RS03950 (791776)	791776..792297	-	522	WP_002916308.1	flavodoxin FldB	-
KWJ12_RS03955 (792398)	792398..793294	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
KWJ12_RS03960 (793317)	793317..794030	+	714	WP_048290928.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KWJ12_RS03965 (794036)	794036..795769	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16035.83 Da
• Isoelectric Point: 11.4778

>T218010 WP_040245400.1 NZ_CP084044:791359-791769 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDSRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T218010 NZ_CP115182:1813507-1814007 [Diaphorobacter sp. ED-3]
GTGACGTTGCGCGCACCGGAGCCGCTGGCCGCGCAGCATCGGCTGGAAGGTTTTGATTGCGGCAAGCCCGCGTTGAATGA
CTGGCTGTTGCGCCACGCCCGCCAGGCGCAGGGCAGCGGCTCGGCCAAGACCTTTGTCGTTGCCGAGGACGATGGCCGCG
TGGCGGGCTACTTCAGTCTGACCGTGGGACAAGTCGACACGCTGGAGGCGCCGGAGCGCATCCGCAAGGGGATGGGGCAG
TACCCATTGCCAGTTGTGATTCTGGCAAGGCTCGCTGTCTCGGTAGCGGATCAGGGACGAGGCATCGGCTTCGGTTTGCT
GCAGGACGCGATTCGCCGCACGATGCTGATTGCCGAGCAGGCCGGCATCCGTGCCATGCTGACCCACCCCATCGATGAGG
AAGCGGCGAGGTTCTACACCCGGTTCGGATTCATCGCGTCACCGCTGCGCGATCAGCAATTGCTACTGCTCTTGAAGGAC
GCCCGTCGCTGGGTCCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT218010 WP_002916312.1 NZ_CP084044:791112-791378 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT218010 NZ_CP115182:1813217-1813510 [Diaphorobacter sp. ED-3]
ATGACTGCCGTCGCATCGTCGCCTTCCGCCCGCTCGGCCCGACTTGGGCTGCGCGCCACCCCCGAACAGGAGGTGGTGCT
GCGCCGTGCCGCCGAGGTGGCCCACAAGTCGCTGACCGACTTCATCCTCGACAGCGCGTGCCTGGCCGCCGAGCAAACCC
TGCTGGACCAACGATTGTTCATGGTCTCGGGTAGCCAGTACCAGGCCCTGATGGATCTGCTGGATCGCCCCGAACAGGCC
AACGAGGATTTGCGCGACCTGTTTGCCCGTAAGGCGCCCTGGGACACGAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41