Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245793..246435 Replicon chromosome
Accession NZ_CP084035
Organism Bacillus sp. 41-22

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag LCF45_RS01385 Protein ID WP_000635965.1
Coordinates 246085..246435 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LCF45_RS01380 Protein ID WP_000004570.1
Coordinates 245793..246080 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF45_RS01355 (LCF45_01355) 241108..242070 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
LCF45_RS01360 (LCF45_01360) 242063..242635 - 573 WP_002124818.1 rhomboid family intramembrane serine protease -
LCF45_RS01365 (LCF45_01365) 242728..243087 + 360 WP_002009969.1 holo-ACP synthase -
LCF45_RS01370 (LCF45_01370) 243244..244194 + 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
LCF45_RS01375 (LCF45_01375) 244312..245481 + 1170 WP_002124821.1 alanine racemase -
LCF45_RS01380 (LCF45_01380) 245793..246080 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LCF45_RS01385 (LCF45_01385) 246085..246435 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LCF45_RS01390 (LCF45_01390) 246504..248672 + 2169 WP_002029442.1 Tex family protein -
LCF45_RS01395 (LCF45_01395) 248730..248846 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LCF45_RS01400 (LCF45_01400) 249057..249512 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T218007 WP_000635965.1 NZ_CP084035:246085-246435 [Bacillus sp. 41-22]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T218007 NZ_CP115176:c961774-961529 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT218007 WP_000004570.1 NZ_CP084035:245793-246080 [Bacillus sp. 41-22]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT218007 NZ_CP115176:c961529-961215 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References