Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 1505020..1505658 Replicon chromosome
Accession NZ_CP084033
Organism Paenibacillus polymyxa strain EB4 G3

Toxin (Protein)


Gene name pemK Uniprot ID H6CFY1
Locus tag LAZ93_RS06370 Protein ID WP_007429335.1
Coordinates 1505308..1505658 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H6CFY0
Locus tag LAZ93_RS06365 Protein ID WP_007429334.1
Coordinates 1505020..1505301 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAZ93_RS06345 (LAZ93_06345) 1500037..1501008 - 972 WP_167440839.1 ABC transporter substrate-binding protein -
LAZ93_RS06350 (LAZ93_06350) 1501243..1501875 + 633 WP_031463107.1 LysE family transporter -
LAZ93_RS06355 (LAZ93_06355) 1501986..1503263 + 1278 WP_031463108.1 outer membrane lipoprotein-sorting protein -
LAZ93_RS06360 (LAZ93_06360) 1503530..1504726 + 1197 WP_031463109.1 alanine racemase -
LAZ93_RS06365 (LAZ93_06365) 1505020..1505301 + 282 WP_007429334.1 ribbon-helix-helix protein, CopG family Antitoxin
LAZ93_RS06370 (LAZ93_06370) 1505308..1505658 + 351 WP_007429335.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LAZ93_RS06375 (LAZ93_06375) 1505837..1508059 + 2223 WP_017426526.1 Tex family protein -
LAZ93_RS06380 (LAZ93_06380) 1508158..1508292 - 135 WP_016821163.1 cortex morphogenetic protein CmpA -
LAZ93_RS06385 (LAZ93_06385) 1508435..1508947 + 513 WP_016821162.1 SprT family protein -
LAZ93_RS06400 (LAZ93_06400) 1509326..1510201 - 876 WP_031463111.1 Cof-type HAD-IIB family hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12822.77 Da        Isoelectric Point: 5.6195

>T218006 WP_007429335.1 NZ_CP084033:1505308-1505658 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Download         Length: 351 bp

>T218006 NZ_CP115175:44859-45122 [Homoserinibacter sp. YIM 151385]
ATGACGCGCCGACTCGTCTGGGTCGACGAGGCCTGGGAGGACTACCTCCACTGGCAGGGGCAGGATCGGAAGACCCTCAA
GCGGATCAACCTGCTCATCGCGGATGTCCGCCGCGGCGATCCCTTCGAGGGGATCGGCAAGCCGGAGCCGCTCAAGCACG
CGCTCGCGGGAGCGTGGTCGCGGCGGATCGACGACGTCAACCGGCTCGTCTACATCGCGGATGCGGAGTCGGTCACGATC
CTCCAGGCGCGATACCACTACTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10636.25 Da        Isoelectric Point: 8.5257

>AT218006 WP_007429334.1 NZ_CP084033:1505020-1505301 [Paenibacillus polymyxa]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Download         Length: 282 bp

>AT218006 NZ_CP115175:44614-44862 [Homoserinibacter sp. YIM 151385]
ATGGCCATCACCGCGAGCGAGGCCCGAGCCGATCTCTTCGGCCTCATCGAGCGCGTCAACCTCGACCACATCGAGGTCGA
GATCACGTCGAAGCGCGGTGCCGCGGTCATCATGTCGAAGGACGAGTACGACGCGCTCATCGAGACCGCTCATCTGCTCC
GATCGCCCGCCAACGCACGTCGACTGCTGCGCGCGCTCGACAGCGCACGGGCCGGCGCGGGCACCGAGCGCGAGCCGATC
GAGGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB H6CFY1

Antitoxin

Source ID Structure
AlphaFold DB H6CFY0

References