Detailed information of TA system TA218000

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1523969..1524768
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: A0A7V8TG63
• Locus tag: LCF41_RS06770
• Protein ID: WP_010296752.1
• Coordinates: 1523969..1524388 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: LCF41_RS06775
• Protein ID: WP_225087399.1
• Coordinates: 1524385..1524768 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS06750	1519034..1519675	+	642	WP_015839564.1	uridine kinase	-
LCF41_RS06755	1519800..1520381	+	582	WP_225087396.1	dCTP deaminase	-
LCF41_RS06760	1520475..1522310	+	1836	WP_225087397.1	outer membrane assembly protein AsmA	-
LCF41_RS06765	1522573..1523925	+	1353	WP_225087398.1	anaerobic C4-dicarboxylate transporter DcuC	-
LCF41_RS06770	1523969..1524388	-	420	WP_010296752.1	DUF86 domain-containing protein	Toxin
LCF41_RS06775	1524385..1524768	-	384	WP_225087399.1	nucleotidyltransferase domain-containing protein	Antitoxin
LCF41_RS06780	1524882..1526468	-	1587	WP_225087400.1	TerC family protein	-
LCF41_RS06785	1527209..1528294	+	1086	WP_225088115.1	UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase	-
LCF41_RS06790	1528418..1529554	+	1137	WP_225087401.1	polysaccharide export protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (4-93)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16052.37 Da
• Isoelectric Point: 6.9682

>T218000 WP_010296752.1 NZ_CP084032:c1524388-1523969 [Pectobacterium colocasium]
MTDILLNKSSTIQRCLRRIREEFDTEEGFRQNFTKQDSVILNIQRACEAAIDIANYLIRIKQLGIPQSSRDSFALLAANQ
IITIDLSDNLQKMVGLRNIAVHDYQALNLDIVIHVISHRLSDFEHFIKQIARYSDSQHL

Nucleotide

• Length: 420 bp

>T218000 NZ_CP115174:c313389-313003 [Sphingomonas sp. PAMB00755]
ATGGTAGGGGCGCTGTTCGACACTAATATCCTGATCGACCACCTGAACGCGATCCCGCAGGCTCGGAAGGAGATCGCGCG
CTACGGCGACCCGGCAATCAGTATCGTCACCTGGATGGAGGTGATGGTCGGGGCCGGCGCCGAATTGGTTGAGCCCACTC
GTCGCTTTCTCGATGGGTTTGCGGTCGTCGAGCTGGACAATGACATTGCCGAGCGCGCTGTTTCGCTTCGTCGCTCTCAT
CGCATCAAGCTTCCGGATGCGATAATCTGGGCGACAGCTCAGGTCGCGGGACGGATCCTGGTAACACGTAACACGAAGGA
TTTTCCGACCGACGATCCGGGGATACGTGAACCCTACGCTATTTGGGGCTCGGTATCGCCTATTTGA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 14354.54 Da
• Isoelectric Point: 4.7463

>AT218000 WP_225087399.1 NZ_CP084032:c1524768-1524385 [Pectobacterium colocasium]
MFDKIVLTLKKQIPDIKIIYLFGSQATGNARADSDIDIAIMATRALDPVERWELSNQLAKEVGHDVDLIDLLQASTVLKM
EIVRNGKLLYDAETAAGEFEMTTLSMYQHLQKERADIIRSFNQDLKA

Nucleotide

• Length: 384 bp

>AT218000 NZ_CP115174:c313598-313383 [Sphingomonas sp. PAMB00755]
ATGCGCGCCCTTGTCGATATGAGTGACAGCCAGGTCGAAGCCCTCGATGTCCTGGCGCGGCGTAAACGCCGCTCCCGCGC
TTCCCTCATTCGTGCGGCGATCGATGATTATCTCGCGCGCCATCGCCACCAGCAGGTCGAGGATGGCTTTGGTTTGTGGG
GTGAGCGCGAGTTGGACGGATTGGCATATCAGGAACAGGTGCGTGGCGAATGGTAG

Structures

Toxin

• AlphaFold DB: A0A7V8TG63