Detailed information of TA system TA217998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1046946..1047457
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LCF41_RS04680
• Protein ID: WP_225087093.1
• Coordinates: 1046946..1047161 (-)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: LCF41_RS04685
• Protein ID: WP_225087094.1
• Coordinates: 1047239..1047457 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS04660	1042674..1043630	+	957	WP_225087089.1	AraC family transcriptional regulator	-
LCF41_RS04665	1043961..1044440	+	480	WP_225087090.1	hypothetical protein	-
LCF41_RS04670	1044620..1044781	-	162	WP_225087091.1	hypothetical protein	-
LCF41_RS04675	1045564..1046550	-	987	WP_225087092.1	SIR2 family protein	-
LCF41_RS04680	1046946..1047161	-	216	WP_225087093.1	CcdB family protein	Toxin
LCF41_RS04685	1047239..1047457	-	219	WP_225087094.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LCF41_RS04690	1047472..1047720	-	249	WP_010299606.1	helix-turn-helix transcriptional regulator	-
LCF41_RS04695	1047805..1048059	-	255	WP_225087095.1	helix-turn-helix transcriptional regulator	-
LCF41_RS04700	1048164..1048406	+	243	WP_225087096.1	helix-turn-helix transcriptional regulator	-
LCF41_RS22230	1048878..1049009	+	132	WP_284144944.1	hypothetical protein	-
LCF41_RS04710	1049241..1051394	+	2154	WP_225087097.1	ornithine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1023790..1048406	24616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (10-70)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 7836.79 Da
• Isoelectric Point: 4.1068

>T217998 WP_225087093.1 NZ_CP084032:c1047161-1046946 [Pectobacterium colocasium]
VYSHTTIEAHDLSDKVNKTLFPQIRINGEDYRLIATELSSVPVEVIGEAIADLGEYADEIKDAINLIFGGF

Nucleotide

• Length: 216 bp

>T217998 NZ_CP115161:c1770266-1770012 [Zunongwangia sp. HRR-M8]
ATGCAAATCACAACTGTTTCTGACTTTAGAAAAGATATCAAAACATACCTGGACAGAGTTATCAAAAACTTTGAAACCTT
AATAATTAACCGCGGAAAAGATTCTGGAATTGTTGTTATGTCTCTAGAAGAATACAATTCTCTAATGGCTACCAATCACG
AATTATCTTCCCGAAAAAATGAATTACGTTTAGATTCTGCTATTGACAAACTAAAAAATGGTAAATCATTTTCAAGAGAC
TTAATCGAAGACTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8264.28 Da
• Isoelectric Point: 7.0130

>AT217998 WP_225087094.1 NZ_CP084032:c1047457-1047239 [Pectobacterium colocasium]
MKRRISIAVDKDDNQIPNAAGINISGLANDVMGKEAHRIKTEVWKKENREGMEEVARFIAQYSSFADENRNW

Nucleotide

• Length: 219 bp

>AT217998 NZ_CP115161:c1770010-1769753 [Zunongwangia sp. HRR-M8]
ATGAAATATACATTTGTTGACGAATCTTGGGAAGATTATTTATATTGGCAAAAAACAGATAAGAAAAAACTCAAAAAAAT
AAACGAACTTTTAAAGGATATATCAAGAAATCCATTTGATGGAATTGGAAAACCAGAACCTTTAAAACATAAATACGCTG
GATTTTGGTCAAGAAGAATTGACAGTGAACATAGATTGATTTATCAATATAAAGAGGGGGAAATTTTAATTGCAAAATGC
AGATTTCATTATGACTAG