Detailed information of TA system TA217997

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 798312..798972
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: LCF41_RS03610
• Protein ID: WP_250160548.1
• Coordinates: 798598..798972 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C6D8Y5
• Locus tag: LCF41_RS03605
• Protein ID: WP_012773340.1
• Coordinates: 798312..798578 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS03585	794282..795286	-	1005	WP_225086920.1	LacI family DNA-binding transcriptional regulator	-
LCF41_RS03590	795341..795949	-	609	WP_225086921.1	HD domain-containing protein	-
LCF41_RS03595	796373..797020	+	648	WP_225086922.1	hemolysin III family protein	-
LCF41_RS03600	797075..798076	-	1002	WP_225086923.1	tRNA-modifying protein YgfZ	-
LCF41_RS03605	798312..798578	+	267	WP_012773340.1	FAD assembly factor SdhE	Antitoxin
LCF41_RS03610	798598..798972	+	375	WP_250160548.1	protein YgfX	Toxin
LCF41_RS03615	799040..799975	+	936	WP_225086925.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
LCF41_RS03620	800045..800287	-	243	WP_225086926.1	hypothetical protein	-
LCF41_RS03625	800316..800834	-	519	WP_225086927.1	flavodoxin FldB	-
LCF41_RS03630	800997..802322	-	1326	WP_225086928.1	MFS transporter	-
LCF41_RS03635	802652..803551	+	900	WP_225086929.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14659.31 Da
• Isoelectric Point: 10.6585

>T217997 WP_250160548.1 NZ_CP084032:798598-798972 [Pectobacterium colocasium]
MQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDRQQLWLASDSMGDDEWRHLRQLLLQQKNWAR

Nucleotide

• Length: 375 bp

>T217997 NZ_CP115161:c1770010-1769753 [Zunongwangia sp. HRR-M8]
ATGAAATATACATTTGTTGACGAATCTTGGGAAGATTATTTATATTGGCAAAAAACAGATAAGAAAAAACTCAAAAAAAT
AAACGAACTTTTAAAGGATATATCAAGAAATCCATTTGATGGAATTGGAAAACCAGAACCTTTAAAACATAAATACGCTG
GATTTTGGTCAAGAAGAATTGACAGTGAACATAGATTGATTTATCAATATAAAGAGGGGGAAATTTTAATTGCAAAATGC
AGATTTCATTATGACTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.94 Da
• Isoelectric Point: 6.2223

>AT217997 WP_012773340.1 NZ_CP084032:798312-798578 [Pectobacterium colocasium]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLSDSDKRTFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT217997 NZ_CP115161:c1770266-1770012 [Zunongwangia sp. HRR-M8]
ATGCAAATCACAACTGTTTCTGACTTTAGAAAAGATATCAAAACATACCTGGACAGAGTTATCAAAAACTTTGAAACCTT
AATAATTAACCGCGGAAAAGATTCTGGAATTGTTGTTATGTCTCTAGAAGAATACAATTCTCTAATGGCTACCAATCACG
AATTATCTTCCCGAAAAAATGAATTACGTTTAGATTCTGCTATTGACAAACTAAAAAATGGTAAATCATTTTCAAGAGAC
TTAATCGAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E501