Detailed information of TA system TA217996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 622437..622963
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LCF41_RS02825
• Protein ID: WP_225086800.1
• Coordinates: 622437..622742 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: LCF41_RS02830
• Protein ID: WP_225086801.1
• Coordinates: 622745..622963 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS02815	619041..621050	+	2010	WP_225086798.1	helicase-related protein	-
LCF41_RS02820	621052..622311	+	1260	WP_225086799.1	GIY-YIG nuclease family protein	-
LCF41_RS02825	622437..622742	-	306	WP_225086800.1	type II toxin-antitoxin system toxin CcdB	Toxin
LCF41_RS02830	622745..622963	-	219	WP_225086801.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LCF41_RS02835	623022..623765	-	744	WP_225086802.1	MobC family replication-relaxation protein	-
LCF41_RS02840	624652..624993	-	342	WP_225086803.1	hypothetical protein	-
LCF41_RS02845	625134..625304	+	171	WP_162847981.1	hypothetical protein	-
LCF41_RS02850	625445..626704	-	1260	WP_225086804.1	integrase arm-type DNA-binding domain-containing protein	-
LCF41_RS02860	627068..627643	-	576	WP_225086805.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	610088..626881	16793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11713.62 Da
• Isoelectric Point: 4.6873

>T217996 WP_225086800.1 NZ_CP084032:c622742-622437 [Pectobacterium colocasium]
MQFIVYEYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSNVPVEVIGEVI
ADLGDCADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T217996 NZ_CP115160:3832103-3832666 [Algoriphagus sp. TR-M9]
ATGATTCATAGCGTCAGATTCATATGCGTCTTAGATACCAATGTCATTTACCCCATAGACATTCGTGATCTGCTTTTTTG
GTTTGCTTTTTATGATCTATTCACCCCAAAATGGAGTAAACATATTTTCGATGAATGGTTGGAGGTGATGATTCGAAAAG
GAGTCAAGCGCACAGAAGCCTTTAAAAGAGTGTCCAAAGCTCACCAGGCATTTCCCGATGCCTTGGTAGAAAACTATGAA
CCAATCATCAGCTCTATAAATCTTCCCGATGAAAAAGACAAGCATGTTCTTGCTGCTGCTATCAAAGCCAATGCAAACAT
TATAGTAACCAATAACCTAAAAGATTTCCCACCTGAGTATTTAGCAACATTTGGCTTAAGTGTGAAAAATGCCGATGACT
TTATAACTGACACTATTGACTTAAATCAAGACCTCGCTCTAAAAGCCTTCAGAACACTAGTCATGAATAGACAAAACCCC
GATTTAAACGAATTTGAAGTCCTCGACAGATTACGCAATTGCGGATTACATGACTCTGCTAACTACTTGCACTCACTTTT
ATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8208.15 Da
• Isoelectric Point: 7.0135

>AT217996 WP_225086801.1 NZ_CP084032:c622963-622745 [Pectobacterium colocasium]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFITQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT217996 NZ_CP115160:3831667-3832113 [Algoriphagus sp. TR-M9]
ATGGAAGATTTGATAAGACCTACCAAAGAAGACCAGAAAGCAGCCATGAAGTCATATGACACGCTCGCATCAATGTTAAA
AAACATTCACTCTGATTACCCTGAAATTGAAATTGAAGAAACCCAAGAGAGAATAAAAATCCCGCTGAATGCTTTAAAAC
TTCTTGCCGAGATTTTAAAGGAAACCAGTCAGGGAAAACCTGTCTCCATAGTCCCAACGGCCACCGAAATAACCACCCAA
GCTGCCGCTGAACTCTTAGGCTGCTCTCGCCCACATTTAGTAAAACTATTAGAGCAAGGAGAAATAAATTTCATCAAAAT
CGGCAAACACCGAAGAATCAAATACGAAGATGTGATTGAGTATAAAAAGAAATTAAAAGCTGAGCAGCGAAGATTACTGA
TCGAGATCATTCAGTCTGACGAAGAATCTGGACTATATGATTCATAG