Detailed information of TA system TA217993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 462208..463852
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LCF41_RS02145
• Protein ID: WP_225086688.1
• Coordinates: 462518..463852 (+)
• Length: 445 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LCF41_RS02140
• Protein ID: WP_225086687.1
• Coordinates: 462208..462513 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS02120	459632..459883	+	252	WP_005973616.1	prevent-host-death protein	-
LCF41_RS02125	459873..460157	+	285	WP_005973612.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LCF41_RS02130	460772..461720	+	949	Protein_411	site-specific integrase	-
LCF41_RS02135	461739..461900	+	162	WP_225086686.1	CopG family transcriptional regulator	-
LCF41_RS02140	462208..462513	+	306	WP_225086687.1	helix-turn-helix transcriptional regulator	Antitoxin
LCF41_RS02145	462518..463852	+	1335	WP_225086688.1	type II toxin-antitoxin system HipA family toxin	Toxin
LCF41_RS02150	464128..465216	-	1089	WP_225088081.1	helicase RepA family protein	-
LCF41_RS02155	465328..466086	-	759	Protein_416	primase-helicase zinc-binding domain-containing protein	-
LCF41_RS02160	466157..466693	-	537	WP_225086689.1	ash family protein	-
LCF41_RS02165	466690..466890	-	201	WP_225086690.1	AlpA family transcriptional regulator	-
LCF41_RS02170	466893..467771	-	879	WP_225086691.1	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	448051..472560	24509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 445 a.a.
• Molecular weight: 50883.00 Da
• Isoelectric Point: 6.0236

>T217993 WP_225086688.1 NZ_CP084032:462518-463852 [Pectobacterium colocasium]
MPRTQQRLAIWMNGIQVGFWEKTRGEDKLQYLPEWIADEQGRPLSLSLLFTPGNQLWRGNVVRDYFDNLLPDSEGIRRRL
ATRYHADSLEPFDLLAELGRDCVGAIQLLNGNEEPTDLFSVKYRPLCEAEIATILRNTTAALLPGRQDDTDDLRLSIAGA
QEKTALLWHERQWCLPEGSTPTTHIFKLPLGLVGNMQADMSTSVENEWLCSLILKEYGIPVAKTQIAQFEDQKVLVVERF
DRRWSSDQQWIIRLPQEDMCQALGVSPLRKYQSDGGPGISDIMTILSHSEQAEQDKAQFFRAQIIFWLIAATDGHAKNFS
IAIEPQGRYHLTPLYDVLSAWPVIGPRNNQISWQKCKLAMAVRGSSNYYHLYRIQRRHWVNHGELTGLGRRQVEAMMDDI
ISRTPEVIERVSSLLPESFPPELAECVFDGMRQQCGRLQELHRK

Nucleotide

• Length: 1335 bp

>T217993 NZ_CP115153:243857-244207 [Sutcliffiella sp. NC1]
TTGATTGTCAAACGCGGTGACGTTTATTTTGCTGATTTATCCCCTGTAGTTGGGTCTGAGCAAGGAGGCGTTCGACCAGT
TCTGGTTATACAAAACGATATTGGTAACCGGTTTAGTCCGACCATTATCGTAGCAGCCATAACAGCACAGATTCAAAAAG
CTAAACTCCCGACACATGTCGAGATTGATGCGAAACGCTATGGCTTTGAACGTGACTCTGTAATATTGTTAGAACAGATT
CGAACCATTGATAAACAAAGATTAACGGACAAAATCACTCATCTTGATGAAGAGATGATGGATCGAGTTGACGAGGCTTT
ACAAATAAGTTTAGGTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11231.01 Da
• Isoelectric Point: 10.7356

>AT217993 WP_225086687.1 NZ_CP084032:462208-462513 [Pectobacterium colocasium]
MNTVYPLKTLNQLRPLLVGFRKAKGLTQKDVSERLGVTQQTYARLEANPGSASIERLFKVLTVLGVEVVLSSGPRVHFSM
SSSTMEEPEKSIGSPARREKW

Nucleotide

• Length: 306 bp

>AT217993 NZ_CP115153:243571-243852 [Sutcliffiella sp. NC1]
GTGTCAGAATCCAGCGCAACAACTGAAATTATGGTTCGATTACCTCAAAACTTAGTATCCGAATTAGATGGATTAGTGAA
ACAGGAAAACGGAAATCGAAATGAGTTAATTTATCAAGCTACAAAAATGTATCTTCGAGAGCGCAAAAAGCGACAAATAC
GTGAATCTATGAGACGTGGCTATATGGAAATGGCAAAGATCAACCTAAATATTGCATCAGAAGCATTTCTAGCAGAATAT
GAGGCAGAGCATACAGTTGAGCGCTTAGTTAGCGGGGGGTAA