Detailed information of TA system TA217992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 459632..460157
• Replicon: chromosome
• Accession: NZ_CP084032
• Organism: Pectobacterium colocasium strain LJ1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1D7Z1B4
• Locus tag: LCF41_RS02125
• Protein ID: WP_005973612.1
• Coordinates: 459873..460157 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C6D956
• Locus tag: LCF41_RS02120
• Protein ID: WP_005973616.1
• Coordinates: 459632..459883 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF41_RS02080	455210..455470	+	261	WP_225086683.1	AlpA family transcriptional regulator	-
LCF41_RS02085	455467..455691	+	225	WP_005973635.1	hypothetical protein	-
LCF41_RS22180	455811..456014	+	204	Protein_403	ash family protein	-
LCF41_RS02095	456050..456247	+	198	WP_225088079.1	host cell division inhibitor Icd-like protein	-
LCF41_RS02100	456240..456434	+	195	WP_225088080.1	hypothetical protein	-
LCF41_RS02105	456431..456697	+	267	WP_225086684.1	hypothetical protein	-
LCF41_RS02110	456694..457035	+	342	WP_212768710.1	DUF5375 family protein	-
LCF41_RS02115	457047..459371	+	2325	WP_225086685.1	phage/plasmid primase, P4 family	-
LCF41_RS02120	459632..459883	+	252	WP_005973616.1	prevent-host-death protein	Antitoxin
LCF41_RS02125	459873..460157	+	285	WP_005973612.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCF41_RS02130	460772..461720	+	949	Protein_411	site-specific integrase	-
LCF41_RS02135	461739..461900	+	162	WP_225086686.1	CopG family transcriptional regulator	-
LCF41_RS02140	462208..462513	+	306	WP_225086687.1	helix-turn-helix transcriptional regulator	-
LCF41_RS02145	462518..463852	+	1335	WP_225086688.1	type II toxin-antitoxin system HipA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	448051..472560	24509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11010.96 Da
• Isoelectric Point: 10.1716

>T217992 WP_005973612.1 NZ_CP084032:459873-460157 [Pectobacterium colocasium]
MSYKLEFEEHALKEFKKLGAPVREQFTKKLKEVLQNPHVPANRLHGMADCYKIKLRSAGYRLVYQVLEHEIVVLVLAVGK
RERSEVYKTAKDRL

Nucleotide

• Length: 285 bp

>T217992 NZ_CP115151:c87021-86578 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9079.35 Da
• Isoelectric Point: 5.0807

>AT217992 WP_005973616.1 NZ_CP084032:459632-459883 [Pectobacterium colocasium]
MSVQPILANAAVSISDFKKSPNAALKEANGEPVAVLTNGRISGYYVSPETWEALADYQEDIELAKIARSRMKGKRVKVDL
DEL

Nucleotide

• Length: 252 bp

>AT217992 NZ_CP115151:c87248-87018 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A1D7Z1B4

Antitoxin

• AlphaFold DB: A0A0G4JQP6