Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 276042..276712 | Replicon | plasmid pKPC2_Vir_090515B1 |
| Accession | NZ_CP084027 | ||
| Organism | Escherichia coli strain 035053C1pKPC2_Vir_090515B1 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q6U619 |
| Locus tag | LC440_RS24405 | Protein ID | WP_004213072.1 |
| Coordinates | 276042..276485 (-) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q6U620 |
| Locus tag | LC440_RS24410 | Protein ID | WP_004213073.1 |
| Coordinates | 276482..276712 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LC440_RS24370 (LC440_24370) | 271452..271727 | + | 276 | WP_004213932.1 | carboxymuconolactone decarboxylase family protein | - |
| LC440_RS24375 (LC440_24375) | 271790..272281 | + | 492 | WP_011251327.1 | DM13 domain-containing protein | - |
| LC440_RS24380 (LC440_24380) | 272330..273250 | + | 921 | WP_004213934.1 | DUF1471 domain-containing protein | - |
| LC440_RS24385 (LC440_24385) | 273341..273744 | + | 404 | Protein_311 | GAF domain-containing protein | - |
| LC440_RS24390 (LC440_24390) | 274263..274898 | - | 636 | Protein_312 | mucoid phenotype regulator RmpA2 | - |
| LC440_RS24395 (LC440_24395) | 275315..275619 | + | 305 | Protein_313 | transposase | - |
| LC440_RS24400 (LC440_24400) | 275642..275893 | - | 252 | WP_186987481.1 | hypothetical protein | - |
| LC440_RS24405 (LC440_24405) | 276042..276485 | - | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LC440_RS24410 (LC440_24410) | 276482..276712 | - | 231 | WP_004213073.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LC440_RS24415 (LC440_24415) | 277320..278453 | + | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| LC440_RS24420 (LC440_24420) | 278469..278762 | + | 294 | WP_004213076.1 | hypothetical protein | - |
| LC440_RS24425 (LC440_24425) | 278752..278958 | - | 207 | WP_004213077.1 | hypothetical protein | - |
| LC440_RS24430 (LC440_24430) | 279310..279600 | + | 291 | WP_004213078.1 | hypothetical protein | - |
| LC440_RS24435 (LC440_24435) | 279590..280489 | + | 900 | WP_004225022.1 | nucleotide-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | dfrA14 / qnrB1 / blaKPC-2 | rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..351436 | 351436 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 16006.74 Da Isoelectric Point: 9.3642
>T217985 WP_004213072.1 NZ_CP084027:c276485-276042 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
Download Length: 444 bp
>T217985 NZ_CP115150:432503-432790 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTAGGTTAACAATCAAACCCTCTGGGGATTAA
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTAGGTTAACAATCAAACCCTCTGGGGATTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.73 Da Isoelectric Point: 4.4563
>AT217985 WP_004213073.1 NZ_CP084027:c276712-276482 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT217985 NZ_CP115150:432194-432496 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|