Detailed information of TA system TA217984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 209729..210465
• Replicon: plasmid pKPC2_Vir_090515B1
• Accession: NZ_CP084027
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: LC440_RS24065
• Protein ID: WP_004098919.1
• Coordinates: 209729..210211 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: LC440_RS24070
• Protein ID: WP_004213599.1
• Coordinates: 210199..210465 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC440_RS24035 (LC440_24035)	205182..205700	+	519	WP_022644913.1	hypothetical protein	-
LC440_RS24040 (LC440_24040)	205784..206488	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LC440_RS24045 (LC440_24045)	206544..206942	+	399	Protein_243	recombinase family protein	-
LC440_RS24050 (LC440_24050)	206989..207639	+	651	WP_015065500.1	SOS response-associated peptidase	-
LC440_RS24055 (LC440_24055)	207936..208931	+	996	WP_019725651.1	hypothetical protein	-
LC440_RS24060 (LC440_24060)	209004..209327	+	324	WP_019725650.1	hypothetical protein	-
LC440_RS24065 (LC440_24065)	209729..210211	-	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
LC440_RS24070 (LC440_24070)	210199..210465	-	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
LC440_RS24075 (LC440_24075)	210660..210890	-	231	WP_004213598.1	hypothetical protein	-
LC440_RS24080 (LC440_24080)	210904..211107	-	204	WP_004213596.1	HHA domain-containing protein	-
LC440_RS24085 (LC440_24085)	211168..211662	-	495	WP_004213594.1	hypothetical protein	-
LC440_RS24090 (LC440_24090)	211704..214673	-	2970	WP_004213592.1	Tn3 family transposase	-
LC440_RS24095 (LC440_24095)	214676..215233	-	558	WP_004213590.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / qnrB1 / blaKPC-2	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2	1..351436	351436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T217984 WP_004098919.1 NZ_CP084027:c210211-209729 [Escherichia coli]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T217984 NZ_CP115150:432194-432496 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT217984 WP_004213599.1 NZ_CP084027:c210465-210199 [Escherichia coli]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT217984 NZ_CP115150:432503-432790 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTAGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A7D3T1D0