Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 209729..210465 Replicon plasmid pKPC2_Vir_090515B1
Accession NZ_CP084027
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LC440_RS24065 Protein ID WP_004098919.1
Coordinates 209729..210211 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LC440_RS24070 Protein ID WP_004213599.1
Coordinates 210199..210465 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC440_RS24035 (LC440_24035) 205182..205700 + 519 WP_022644913.1 hypothetical protein -
LC440_RS24040 (LC440_24040) 205784..206488 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LC440_RS24045 (LC440_24045) 206544..206942 + 399 Protein_243 recombinase family protein -
LC440_RS24050 (LC440_24050) 206989..207639 + 651 WP_015065500.1 SOS response-associated peptidase -
LC440_RS24055 (LC440_24055) 207936..208931 + 996 WP_019725651.1 hypothetical protein -
LC440_RS24060 (LC440_24060) 209004..209327 + 324 WP_019725650.1 hypothetical protein -
LC440_RS24065 (LC440_24065) 209729..210211 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LC440_RS24070 (LC440_24070) 210199..210465 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LC440_RS24075 (LC440_24075) 210660..210890 - 231 WP_004213598.1 hypothetical protein -
LC440_RS24080 (LC440_24080) 210904..211107 - 204 WP_004213596.1 HHA domain-containing protein -
LC440_RS24085 (LC440_24085) 211168..211662 - 495 WP_004213594.1 hypothetical protein -
LC440_RS24090 (LC440_24090) 211704..214673 - 2970 WP_004213592.1 Tn3 family transposase -
LC440_RS24095 (LC440_24095) 214676..215233 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / qnrB1 / blaKPC-2 rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..351436 351436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T217984 WP_004098919.1 NZ_CP084027:c210211-209729 [Escherichia coli]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T217984 NZ_CP115150:432194-432496 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT217984 WP_004213599.1 NZ_CP084027:c210465-210199 [Escherichia coli]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT217984 NZ_CP115150:432503-432790 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTAGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References