Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 85588..86231 Replicon plasmid pKPC2_Vir_090515B1
Accession NZ_CP084027
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag LC440_RS23310 Protein ID WP_032425563.1
Coordinates 85588..86004 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LC440_RS23315 Protein ID WP_001261276.1
Coordinates 86001..86231 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC440_RS23295 (LC440_23295) 80632..82740 - 2109 WP_032427226.1 peptidase domain-containing ABC transporter -
LC440_RS23300 (LC440_23300) 82730..84007 - 1278 WP_009309915.1 HlyD family secretion protein -
LC440_RS23305 (LC440_23305) 84669..85547 + 879 WP_064182004.1 restriction endonuclease -
LC440_RS23310 (LC440_23310) 85588..86004 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
LC440_RS23315 (LC440_23315) 86001..86231 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LC440_RS23320 (LC440_23320) 86819..87763 + 945 WP_011977810.1 hypothetical protein -
LC440_RS23325 (LC440_23325) 87800..88192 + 393 WP_011977811.1 hypothetical protein -
LC440_RS23330 (LC440_23330) 88250..88771 + 522 WP_013214008.1 hypothetical protein -
LC440_RS23335 (LC440_23335) 88817..89020 + 204 WP_011977813.1 hypothetical protein -
LC440_RS23340 (LC440_23340) 89050..90054 + 1005 WP_011977814.1 hypothetical protein -
LC440_RS23345 (LC440_23345) 90238..91017 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / qnrB1 / blaKPC-2 rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..351436 351436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T217983 WP_032425563.1 NZ_CP084027:c86004-85588 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T217983 NZ_CP114976:c2606140-2605859 [Denitrificimonas caeni]
ATGTACCAGTTAGGTTTTGGCCGTCAGGCGCGTAAAGAGTGGGATAAGCTGAGCAAACCTATTCAGCTGCAGTTTGCTCT
AAAGCTGAAAGAGCGACTGAAAAATCCACATGTTAAAAAAGATGCGCTAAGTGGTATGCCGCATTGCTACAAGATTAAGT
TACGCAGCATAGGTTTTCGTTTGGTCTATCAGGTTTTGGATCAGACGCTGGTTATCACAGTGGTGGCGGTAGGTAAGCGT
GAGCGCAACGAGGTTTATCGTAAAGCGCGGGAGCGTCTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT217983 WP_001261276.1 NZ_CP084027:c86231-86001 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT217983 NZ_CP114976:c2606429-2606142 [Denitrificimonas caeni]
ATGAACATGGTGCAACTCACACCTTTATTGGCTGATTTATCAGCATCAGTGACTGAGCTAAAAAAAGACCCCATGGGAAT
TATTCGCAGTGGGTTGGGGGAGACTGTGGTTATTCTTAACCGCAATGAGCCTGCTTTTTATGCTGTACCCCCAGCACAGT
ACGAGCTAATGATGCAGTTGATTGATGATGCGCACTTGGCGCAGCTTGTTAAGCAGCGGCAGGACGATCCAGTGGAGATG
GTTGATATTGACGACCTTATCCAGCAAGCCAGCAGTATCAGTAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References