Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 38804..39714 Replicon plasmid pKPC2_Vir_090515B1
Accession NZ_CP084027
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LC440_RS23060 Protein ID WP_004026354.1
Coordinates 39244..39714 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag LC440_RS23055 Protein ID WP_004026357.1
Coordinates 38804..39247 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC440_RS23025 (LC440_23025) 33822..34343 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
LC440_RS23030 (LC440_23030) 34340..35293 + 954 Protein_40 fec operon regulator FecR -
LC440_RS23035 (LC440_23035) 35380..37506 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LC440_RS23040 (LC440_23040) 37581..37700 + 120 Protein_42 Fe3+-citrate ABC transporter substrate-binding protein -
LC440_RS23045 (LC440_23045) 37881..38102 + 222 WP_004213643.1 DUF2188 domain-containing protein -
LC440_RS23050 (LC440_23050) 38200..38703 + 504 Protein_44 DUF4113 domain-containing protein -
LC440_RS23055 (LC440_23055) 38804..39247 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
LC440_RS23060 (LC440_23060) 39244..39714 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LC440_RS23065 (LC440_23065) 40160..40857 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
LC440_RS23070 (LC440_23070) 42244..42894 - 651 WP_068893702.1 DUF1173 family protein -
LC440_RS23075 (LC440_23075) 42927..43193 - 267 WP_223175074.1 DUF1173 family protein -
LC440_RS23080 (LC440_23080) 43371..43865 + 495 WP_004212794.1 thermonuclease family protein -
LC440_RS23085 (LC440_23085) 44202..44600 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / qnrB1 / blaKPC-2 rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA2 1..351436 351436
- flank IS/Tn - - 40354..40857 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T217982 WP_004026354.1 NZ_CP084027:39244-39714 [Escherichia coli]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T217982 NZ_CP114976:c2003671-2003369 [Denitrificimonas caeni]
ATGGCTGAAATAATCTGGACGGAGCCAGCCCTTCATGAACTGGATGCTCTCGCTGAGTATGTAGCGTTGGATAATCCTGA
AGCGGCCAGCCATTTGGTCGAAAAAGTGTTTGATAAAATTGAGAGTCTAGAGAATTTTCCCCAGTCTGGACGGGTTCCTC
CAGGACTGCCCAATTCCGTATACAGGGAGGTAGTAGTGACTCCGTGTCGCATTTTTTATCGAGAAGATGATAAGCGGGTT
CTTATCCTATATGTCATGCGAGAGGAGCGGCAGCTTCGTGCTTACATGCTGGAGAGCAGCTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT217982 WP_004026357.1 NZ_CP084027:38804-39247 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT217982 NZ_CP114976:c2003916-2003659 [Denitrificimonas caeni]
ATGAAAGTTGAGCTTGTTACAAACCTTAAGCGCCAAGCCACAAAAATCTTGGCAGATCTGCGTTTGTCCAAAGAACCGGT
ACTGATCACTGAACATGGTCAGCCATCCGCTTATCTCGTTGATGTGCAGGATTACGAGTTTATGCAGCGTCGGCTTGAAC
TGCTTGAGGGGCTCTCACGGGGAGAGCGCGCTGTGCTTGAGGGAAGAACGTACAGCCAAAGTGAGGCTAGGGAGAAAATG
AGTAAATGGCTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References