Detailed information of TA system TA021798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 163531..164170
• Replicon: chromosome
• Accession: NC_008611
• Organism: Mycobacterium ulcerans Agy99

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MUL_RS01230
• Protein ID: WP_011738541.1
• Coordinates: 163531..163977 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: B2HEJ8
• Locus tag: MUL_RS01235
• Protein ID: WP_011738542.1
• Coordinates: 163982..164170 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUL_RS01205	158704..160224	+	1521	WP_011738537.1	carotenoid oxygenase family protein	-
MUL_RS01210	160241..160714	+	474	WP_071498317.1	VOC family protein	-
MUL_RS01215	160720..161154	-	435	WP_011738539.1	hypothetical protein	-
MUL_RS01220	161225..161998	-	774	WP_011738540.1	VOC family protein	-
MUL_RS01225	162164..163419	+	1256	Protein_159	hypothetical protein	-
MUL_RS01230	163531..163977	-	447	WP_011738541.1	SRPBCC family protein	Toxin
MUL_RS01235	163982..164170	-	189	WP_011738542.1	antitoxin	Antitoxin
MUL_RS26255	164272..164894	-	623	Protein_162	hypothetical protein	-
MUL_RS26260	165057..165557	-	501	Protein_163	hypothetical protein	-
MUL_RS01245	165556..166890	+	1335	WP_011738543.1	IS256 family transposase	-
MUL_RS01250	166947..167180	+	234	Protein_165	IS256 family transposase	-
MUL_RS01255	167257..168244	-	988	Protein_166	ISAs1 family transposase	-
MUL_RS26265	168244..168499	-	256	Protein_167	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15808.38 Da
• Isoelectric Point: 7.3597

>T21798 WP_011738541.1 NC_008611:c163977-163531 [Mycobacterium ulcerans Agy99]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLPETLEKGAVVESIVQVKGMHNRIKWTIVCYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEEGSIVSFDVHLGGPALFGPIGMVVAAALRSDINASLRNFVTVFAPPAAG

Nucleotide

• Length: 447 bp

>T21798 NC_008611:c163977-163531 [Mycobacterium ulcerans Agy99]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGTGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGATCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCAGCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGCCGGCTGCCGGCTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6586.35 Da
• Isoelectric Point: 4.7205

>AT21798 WP_011738542.1 NC_008611:c164170-163982 [Mycobacterium ulcerans Agy99]
MGILDKAKDLLAQNADKVETVIDKAGEFVDEKTEGKYSDTIHKVQEGAKKVAGPGAAGEQQN

Nucleotide

• Length: 189 bp

>AT21798 NC_008611:c164170-163982 [Mycobacterium ulcerans Agy99]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAAGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LB02