Detailed information of TA system TA217977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3894814..3895628
• Replicon: chromosome
• Accession: NZ_CP084026
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LC440_RS19125
• Protein ID: WP_001054376.1
• Coordinates: 3894814..3895071 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LC440_RS19130
• Protein ID: WP_001309181.1
• Coordinates: 3895083..3895628 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC440_RS19100 (3890102)	3890102..3891208	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
LC440_RS19105 (3891273)	3891273..3892253	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LC440_RS19110 (3892363)	3892363..3892568	+	206	Protein_3735	HNH endonuclease	-
LC440_RS19115 (3892836)	3892836..3894076	-	1241	Protein_3736	helicase YjhR	-
LC440_RS19120 (3894192)	3894192..3894323	+	132	WP_001309182.1	hypothetical protein	-
LC440_RS19125 (3894814)	3894814..3895071	+	258	WP_001054376.1	YjhX family toxin	Toxin
LC440_RS19130 (3895083)	3895083..3895628	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LC440_RS19135 (3895684)	3895684..3896430	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LC440_RS19140 (3896599)	3896599..3896817	+	219	Protein_3741	hypothetical protein	-
LC440_RS19145 (3896855)	3896855..3896971	+	117	Protein_3742	VOC family protein	-
LC440_RS19150 (3897216)	3897216..3898337	+	1122	WP_000010829.1	M42 family metallopeptidase	-
LC440_RS19155 (3898334)	3898334..3898612	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LC440_RS19160 (3898624)	3898624..3899937	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3884600..3903852	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T217977 WP_001054376.1 NZ_CP084026:3894814-3895071 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T217977 NZ_CP114896:4280509-4280907 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT217977 WP_001309181.1 NZ_CP084026:3895083-3895628 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT217977 NZ_CP114896:4280213-4280506 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8