Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3318389..3319007 Replicon chromosome
Accession NZ_CP084026
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LC440_RS16345 Protein ID WP_001291435.1
Coordinates 3318789..3319007 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LC440_RS16340 Protein ID WP_000344800.1
Coordinates 3318389..3318763 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC440_RS16330 (3313478) 3313478..3314671 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LC440_RS16335 (3314694) 3314694..3317843 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LC440_RS16340 (3318389) 3318389..3318763 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LC440_RS16345 (3318789) 3318789..3319007 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LC440_RS16350 (3319179) 3319179..3319730 + 552 WP_000102564.1 maltose O-acetyltransferase -
LC440_RS16355 (3319846) 3319846..3320316 + 471 WP_000136192.1 YlaC family protein -
LC440_RS16360 (3320480) 3320480..3322030 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LC440_RS16365 (3322072) 3322072..3322425 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LC440_RS16375 (3322804) 3322804..3323115 + 312 WP_000409911.1 MGMT family protein -
LC440_RS16380 (3323146) 3323146..3323718 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T217972 WP_001291435.1 NZ_CP084026:3318789-3319007 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T217972 NZ_CP114896:c3162482-3162375 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT217972 WP_000344800.1 NZ_CP084026:3318389-3318763 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT217972 NZ_CP114896:3162530-3162596 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References