Detailed information of TA system TA217971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2237855..2238493
• Replicon: chromosome
• Accession: NZ_CP084026
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LC440_RS10910
• Protein ID: WP_000813794.1
• Coordinates: 2238317..2238493 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LC440_RS10905
• Protein ID: WP_001270286.1
• Coordinates: 2237855..2238271 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC440_RS10885 (2233007)	2233007..2233948	-	942	WP_001251304.1	ABC transporter permease	-
LC440_RS10890 (2233949)	2233949..2234962	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LC440_RS10895 (2234980)	2234980..2236125	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
LC440_RS10900 (2236370)	2236370..2237776	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
LC440_RS10905 (2237855)	2237855..2238271	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LC440_RS10910 (2238317)	2238317..2238493	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LC440_RS10915 (2238715)	2238715..2238945	+	231	WP_000494244.1	YncJ family protein	-
LC440_RS10920 (2239037)	2239037..2240998	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LC440_RS10925 (2241071)	2241071..2241607	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LC440_RS10930 (2241699)	2241699..2242691	+	993	Protein_2137	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T217971 WP_000813794.1 NZ_CP084026:c2238493-2238317 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T217971 NZ_CP114896:2739731-2740195 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT217971 WP_001270286.1 NZ_CP084026:c2238271-2237855 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT217971 NZ_CP114896:2739396-2739731 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 6HPB