Detailed information of TA system TA217960

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1667936..1668767
• Replicon: chromosome
• Accession: NZ_CP084026
• Organism: Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: LC440_RS08005
• Protein ID: WP_000854814.1
• Coordinates: 1667936..1668310 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: LC440_RS08010
• Protein ID: WP_001285584.1
• Coordinates: 1668399..1668767 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC440_RS07965 (1663332)	1663332..1664498	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
LC440_RS07970 (1664617)	1664617..1665090	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
LC440_RS07975 (1665288)	1665288..1666346	+	1059	WP_001200891.1	FUSC family protein	-
LC440_RS07980 (1666518)	1666518..1666847	+	330	WP_000450409.1	DUF496 family protein	-
LC440_RS07985 (1666948)	1666948..1667082	-	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
LC440_RS07990 (1667202)	1667202..1667330	+	129	Protein_1560	transposase domain-containing protein	-
LC440_RS07995 (1667619)	1667619..1667699	-	81	Protein_1561	hypothetical protein	-
LC440_RS08000 (1667745)	1667745..1667939	-	195	WP_000988600.1	DUF5983 family protein	-
LC440_RS08005 (1667936)	1667936..1668310	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
LC440_RS08010 (1668399)	1668399..1668767	-	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LC440_RS08015 (1668841)	1668841..1669062	-	222	WP_000692323.1	DUF987 domain-containing protein	-
LC440_RS08020 (1669125)	1669125..1669571	-	447	WP_000187523.1	RadC family protein	-
LC440_RS08025 (1669568)	1669568..1671100	-	1533	WP_001350525.1	protein YeeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T217960 WP_000854814.1 NZ_CP084026:c1668310-1667936 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T217960 NZ_CP114896:c1101173-1100886 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT217960 WP_001285584.1 NZ_CP084026:c1668767-1668399 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT217960 NZ_CP114896:c1101412-1101173 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6