Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 965634..966301 Replicon chromosome
Accession NZ_CP084026
Organism Escherichia coli strain 035053C1pKPC2_Vir_090515B1

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag LC440_RS04705 Protein ID WP_001094400.1
Coordinates 965634..965963 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag LC440_RS04710 Protein ID WP_000072690.1
Coordinates 965984..966301 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC440_RS04700 (960690) 960690..965270 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
LC440_RS04705 (965634) 965634..965963 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
LC440_RS04710 (965984) 965984..966301 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LC440_RS04715 (966339) 966339..966539 - 201 WP_001395452.1 DUF987 domain-containing protein -
LC440_RS04720 (966548) 966548..967030 - 483 WP_001407480.1 RadC family protein -
LC440_RS04725 (967039) 967039..967497 - 459 WP_000211841.1 antirestriction protein -
LC440_RS04730 (967600) 967600..967784 - 185 Protein_920 DUF905 domain-containing protein -
LC440_RS04735 (968395) 968395..970098 - 1704 WP_000896263.1 protein YfjW -
LC440_RS04740 (970240) 970240..971249 + 1010 Protein_922 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - luxS 911300..987257 75957
- flank IS/Tn - - 959435..960643 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T217957 WP_001094400.1 NZ_CP084026:c965963-965634 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T217957 NZ_CP114896:c869238-869044 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT217957 WP_000072690.1 NZ_CP084026:c966301-965984 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT217957 NZ_CP114896:868867-869045 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References