Detailed information of TA system TA217949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4329706..4331350
• Replicon: chromosome
• Accession: NZ_CP084023
• Organism: Pectobacterium aroidearum strain LJ2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LCF43_RS19475
• Protein ID: WP_181846230.1
• Coordinates: 4329706..4331040 (-)
• Length: 445 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LCF43_RS19480
• Protein ID: WP_181846231.1
• Coordinates: 4331045..4331350 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF43_RS19450	4325787..4326665	+	879	WP_225181586.1	integrase domain-containing protein	-
LCF43_RS19455	4326668..4326868	+	201	WP_014698606.1	AlpA family transcriptional regulator	-
LCF43_RS19460	4326865..4327401	+	537	WP_225181587.1	ash family protein	-
LCF43_RS19465	4327394..4328230	+	837	Protein_3807	toprim domain-containing protein	-
LCF43_RS19470	4328342..4329430	+	1089	WP_225182648.1	helicase RepA family protein	-
LCF43_RS19475	4329706..4331040	-	1335	WP_181846230.1	type II toxin-antitoxin system HipA family toxin	Toxin
LCF43_RS19480	4331045..4331350	-	306	WP_181846231.1	helix-turn-helix transcriptional regulator	Antitoxin
LCF43_RS19485	4331495..4331701	+	207	WP_225181588.1	hypothetical protein	-
LCF43_RS19490	4331827..4333083	-	1257	WP_225181589.1	tyrosine-type recombinase/integrase	-
LCF43_RS19500	4333617..4334693	-	1077	WP_181829931.1	LPS export ABC transporter permease LptG	-
LCF43_RS19505	4334693..4335808	-	1116	WP_181829932.1	LPS export ABC transporter permease LptF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4308470..4331808	23338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-68)

Sequences

Toxin

Protein

• Length: 445 a.a.
• Molecular weight: 50744.84 Da
• Isoelectric Point: 6.0703

>T217949 WP_181846230.1 NZ_CP084023:c4331040-4329706 [Pectobacterium aroidearum]
MPRTQQRLAIWMNGIQVGFWEKTRGEDRLQYLPEWIADEQGRPLSLSLLFTPGNQIWRGNVVRDYFDNLLPDSEGIRRRL
ATRYHADSLEPFDLLAELGRDCVGAIQLLNGNEEPTDLFSVKYRPLCDAEIAAILRNTTAALLPGRQDDTDDLRLSIAGA
QEKTALLWHEGQWCLPEGSTPTTHIFKLPLGLVGNMQADMSTSVENEWLCSLILKEYGIPVAKTQIAQFEEQKALVVERF
DRRWSSDRQWIIRLPQEDMCQALGVSPLRKYQSDGGPGISDIMTILSHSEQAEQDKAQFFRAQIIFWLIAATDGHAKNFS
IAIEPQGRYHLTPLYDVLSAWPVIGPRNNQISWQKCKLAMAVRGSSNYYHLYRIKRRHWVNHGELTGLGRRQAEAMMDDI
ISRTPEVIERVSSLLPESFPPELAECVFDGMRQQCGRLQELQRK

Nucleotide

• Length: 1335 bp

>T217949 NZ_CP114895:c3684114-3683707 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11258.97 Da
• Isoelectric Point: 10.3119

>AT217949 WP_181846231.1 NZ_CP084023:c4331350-4331045 [Pectobacterium aroidearum]
MNTVYPLKTLNQLRPLLIGFRKAKGLTQKDVSERLGVTQQTYARLEANPGSASIERLFKVLTVLGVEVVLSSGPNVHFSM
SSSTMEEPEKPTDSPARREKW

Nucleotide

• Length: 306 bp

>AT217949 NZ_CP114895:c3684361-3684095 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA