Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3574946..3575571 Replicon chromosome
Accession NZ_CP084023
Organism Pectobacterium aroidearum strain LJ2

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag LCF43_RS16250 Protein ID WP_005976087.1
Coordinates 3575368..3575571 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID C6DB73
Locus tag LCF43_RS16245 Protein ID WP_005976089.1
Coordinates 3574946..3575314 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF43_RS16230 3572453..3573646 - 1194 WP_181848129.1 IS4 family transposase -
LCF43_RS16235 3573940..3574188 + 249 WP_015839361.1 type B 50S ribosomal protein L31 -
LCF43_RS16240 3574206..3574349 + 144 WP_005976091.1 type B 50S ribosomal protein L36 -
LCF43_RS16245 3574946..3575314 + 369 WP_005976089.1 Hha toxicity modulator TomB Antitoxin
LCF43_RS16250 3575368..3575571 + 204 WP_005976087.1 HHA domain-containing protein Toxin
LCF43_RS16260 3576318..3576638 + 321 WP_015839360.1 MGMT family protein -
LCF43_RS16265 3576658..3577239 - 582 WP_225181329.1 YbaY family lipoprotein -
LCF43_RS16270 3577452..3578315 + 864 WP_225181330.1 acyl-CoA thioesterase II -
LCF43_RS16275 3578468..3579754 - 1287 WP_015839357.1 ammonium transporter AmtB -
LCF43_RS16280 3579792..3580130 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3572453..3573646 1193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T217945 WP_005976087.1 NZ_CP084023:3575368-3575571 [Pectobacterium aroidearum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T217945 NZ_CP114895:c2730144-2729893 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14032.77 Da        Isoelectric Point: 4.4787

>AT217945 WP_005976089.1 NZ_CP084023:3574946-3575314 [Pectobacterium aroidearum]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Download         Length: 369 bp

>AT217945 NZ_CP114895:c2729896-2729642 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7Z3G5

References