Detailed information of TA system TA217944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3090195..3090748
• Replicon: chromosome
• Accession: NZ_CP084023
• Organism: Pectobacterium aroidearum strain LJ2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LCF43_RS14135
• Protein ID: WP_119880390.1
• Coordinates: 3090195..3090509 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6CEY1
• Locus tag: LCF43_RS14140
• Protein ID: WP_012769207.1
• Coordinates: 3090512..3090748 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF43_RS14110	3085537..3086648	+	1112	WP_225181186.1	IS3 family transposase	-
LCF43_RS14115	3086886..3087761	+	876	WP_225181187.1	hypothetical protein	-
LCF43_RS14120	3087961..3088563	-	603	WP_065684227.1	site-specific integrase	-
LCF43_RS14125	3088746..3089711	-	966	WP_006326560.1	hypothetical protein	-
LCF43_RS14130	3089979..3090140	+	162	Protein_2766	mobilization protein mobC	-
LCF43_RS14135	3090195..3090509	-	315	WP_119880390.1	CcdB family protein	Toxin
LCF43_RS14140	3090512..3090748	-	237	WP_012769207.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LCF43_RS14145	3091068..3092015	-	948	Protein_2769	zincin-like metallopeptidase domain-containing protein	-
LCF43_RS14150	3092789..3095098	+	2310	WP_205946791.1	TerB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3056525..3100373	43848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11496.46 Da
• Isoelectric Point: 6.9727

>T217944 WP_119880390.1 NZ_CP084023:c3090509-3090195 [Pectobacterium aroidearum]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVMLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T217944 NZ_CP114895:c2729896-2729642 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9013.03 Da
• Isoelectric Point: 6.4631

>AT217944 WP_012769207.1 NZ_CP084023:c3090748-3090512 [Pectobacterium aroidearum]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT217944 NZ_CP114895:c2730144-2729893 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E4H0