Detailed information of TA system TA217943

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1555688..1556413
• Replicon: chromosome
• Accession: NZ_CP084023
• Organism: Pectobacterium aroidearum strain LJ2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LCF43_RS07045
• Protein ID: WP_225182226.1
• Coordinates: 1555688..1556008 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LCF43_RS07050
• Protein ID: WP_225182227.1
• Coordinates: 1556078..1556413 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCF43_RS07020	1551791..1552276	+	486	WP_014699424.1	Hcp family type VI secretion system effector	-
LCF43_RS07025	1552434..1552631	+	198	WP_225182224.1	hypothetical protein	-
LCF43_RS07030	1552719..1553351	-	633	WP_225182225.1	hypothetical protein	-
LCF43_RS07035	1553487..1554053	-	567	Protein_1366	integrase arm-type DNA-binding domain-containing protein	-
LCF43_RS07040	1554744..1555577	-	834	WP_225182591.1	DUF4942 domain-containing protein	-
LCF43_RS07045	1555688..1556008	-	321	WP_225182226.1	TA system toxin CbtA family protein	Toxin
LCF43_RS07050	1556078..1556413	-	336	WP_225182227.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LCF43_RS07055	1556453..1556926	-	474	WP_225182228.1	DNA repair protein RadC	-
LCF43_RS07060	1557054..1557404	-	351	WP_039345641.1	hypothetical protein	-
LCF43_RS07065	1557461..1558294	-	834	WP_225182229.1	hypothetical protein	-
LCF43_RS07070	1558441..1558599	-	159	WP_154715508.1	hypothetical protein	-
LCF43_RS07075	1558785..1559672	-	888	WP_225182230.1	50S ribosome-binding GTPase	-
LCF43_RS07080	1559781..1560725	-	945	WP_225182231.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1543622..1571957	28335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12105.82 Da
• Isoelectric Point: 5.0744

>T217943 WP_225182226.1 NZ_CP084023:c1556008-1555688 [Pectobacterium aroidearum]
MQTTPAIPPREDNPCASHIAVWQQLLTYLLEKHYGLSLNDTPFCEENVIQEHIDAGITLLNAVNFLVEKYELVRIDRNGF
NWQEQSPFITTVDILRARRAAGLLKA

Nucleotide

• Length: 321 bp

>T217943 NZ_CP114895:2718000-2718374 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12385.02 Da
• Isoelectric Point: 6.0676

>AT217943 WP_225182227.1 NZ_CP084023:c1556413-1556078 [Pectobacterium aroidearum]
MSSSDTPEWGLKCAITPRFGARLVQEGNRLHYLVDRASFIGTFSKAEARNLEHVFPELIKQLEQKLRTGELNPHQQGCVT
LHCTEWTCEADTLGSVGYVYIVIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT217943 NZ_CP114895:2717543-2717911 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA