Detailed information of TA system TA217908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 207532..208442
• Replicon: plasmid pKP2648-VirV
• Accession: NZ_CP083992
• Organism: Escherichia coli strain EC2648-Vir2

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LCH23_RS23690
• Protein ID: WP_004026354.1
• Coordinates: 207532..208002 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LCH23_RS23695
• Protein ID: WP_004026357.1
• Coordinates: 207999..208442 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCH23_RS23665 (LCH23_23675)	202944..203342	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
LCH23_RS23670 (LCH23_23680)	203679..204173	-	495	WP_004212794.1	thermonuclease family protein	-
LCH23_RS23675 (LCH23_23685)	204351..204617	+	267	WP_223175074.1	DUF1173 family protein	-
LCH23_RS23680 (LCH23_23690)	204650..205300	+	651	WP_068893702.1	DUF1173 family protein	-
LCH23_RS23690 (LCH23_23700)	207532..208002	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LCH23_RS23695 (LCH23_23705)	207999..208442	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LCH23_RS23700 (LCH23_23710)	208543..209046	-	504	Protein_221	DUF4113 domain-containing protein	-
LCH23_RS23705 (LCH23_23715)	209252..210232	-	981	WP_250684767.1	IS5-like element IS5 family transposase	-
LCH23_RS23710 (LCH23_23720)	210298..211605	+	1308	Protein_223	FepA family TonB-dependent siderophore receptor	-
LCH23_RS23715 (LCH23_23725)	211667..211888	+	222	WP_004213615.1	hypothetical protein	-
LCH23_RS23720 (LCH23_23730)	212031..212933	-	903	WP_004213613.1	DMT family inner membrane transporter PEG344	-
LCH23_RS23725 (LCH23_23735)	213006..213407	-	402	WP_214588034.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA	1..222599	222599
	-	inside	IScluster/Tn	-	-	201490..210232	8742

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T217908 WP_004026354.1 NZ_CP083992:c208002-207532 [Escherichia coli]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T217908 NZ_CP114894:c997173-996886 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT217908 WP_004026357.1 NZ_CP083992:c208442-207999 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT217908 NZ_CP114894:c997412-997173 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9