Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4130616..4131113 Replicon chromosome
Accession NZ_CP083990
Organism Escherichia coli strain EC2648-Vir2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LCH23_RS20175 Protein ID WP_001173343.1
Coordinates 4130616..4130789 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LCH23_RS20180 Protein ID WP_000398619.1
Coordinates 4130817..4131113 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH23_RS20165 (4127286) 4127286..4128743 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LCH23_RS20170 (4128980) 4128980..4130497 + 1518 WP_001295074.1 lysine--tRNA ligase -
LCH23_RS20175 (4130616) 4130616..4130789 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LCH23_RS20180 (4130817) 4130817..4131113 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LCH23_RS20185 (4131340) 4131340..4131612 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
LCH23_RS20190 (4131624) 4131624..4131854 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LCH23_RS20195 (4132035) 4132035..4133666 + 1632 WP_001216477.1 sensor histidine kinase -
LCH23_RS20200 (4133663) 4133663..4134382 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T217905 WP_001173343.1 NZ_CP083990:c4130789-4130616 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T217905 NZ_CP114894:c765237-765043 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT217905 WP_000398619.1 NZ_CP083990:c4131113-4130817 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT217905 NZ_CP114894:764866-765044 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References